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A kind of per2 protein agonist polypeptide and its application

An agonist and protein technology, applied in the direction of peptide/protein components, peptides, medical preparations containing active ingredients, etc., can solve problems such as drugs that have not yet been developed for esophageal cancer, and achieve the effect of increasing the survival rate

Active Publication Date: 2016-09-28
广州誉嘉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no drug for the treatment of esophageal cancer that has developed a mature PER2 protein agonist polypeptide

Method used

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  • A kind of per2 protein agonist polypeptide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The effect of PER2 protein agonist polypeptide on the proliferation of human esophageal cancer cell TE1.

[0017] The MTT colorimetric method was used. Add 1.0×105 logarithmically grown TE1 cells into a 96-well culture plate and culture for 24 hours. Add different concentrations of experimental drug PER2 protein agonist polypeptide and positive control drug vincristine to the experimental wells and positive drug control wells; blank The same volume of solvent was added to each group. Set five duplicate wells in each well, culture for 48h, add MTT to each well at 0h, 2h, 8h, 14h, 20h, 24h, 36h, and 48h respectively, add DMSO after 4h of action, incubate for 30min, and measure at 620nm in a microplate reader Absorbance A value, according to the formula tumor cell growth inhibition rate = (1-absorbance value of the experimental group / absorbance value of the control group) × 100%. The calculated IC50 of the test drug was 6.40 μM. When the concentration is 6.40μM, the inh...

Embodiment 2

[0019] The effect of PER2 protein agonist polypeptide on the proliferation of human esophageal cancer cell ECA109.

[0020] The MTT colorimetric method was used. ECA109 cells with logarithmic growth were added to 96-well culture plate at 1.0×105, and cultured for 24 hours. Different concentrations of experimental drug PER2 protein agonist polypeptide and positive control drug vincristine were added to the experimental wells and positive drug control wells; blank The same volume of solvent was added to each group. Set five duplicate wells in each well, culture for 48h, add MTT to each well at 0h, 2h, 8h, 14h, 20h, 24h, 36h, and 48h respectively, add DMSO after 4h of action, incubate for 30min, and measure at 620nm in a microplate reader Absorbance A value, according to the formula tumor cell growth inhibition rate = (1-absorbance value of the experimental group / absorbance value of the control group) × 100%. The calculated IC50 of the test drug was 7.13 μM. When the concentra...

Embodiment 3

[0022] The in vivo activity of the PER2 protein agonist polypeptide was tested with a tumor model.

[0023] The Eca109 tumor model was established, and the positive control drug vincristine was added; the same volume of solvent was added to the blank group, and three doses were set in the experimental group: 6, 12, and 24 mg / Kg. After 21 days, the number of surviving mice was observed, and the survival rate was calculated. The results showed that the PER2 protein agonist polypeptide can effectively protect mice and improve the survival rate of tumor-bearing mice, with a survival rate of 78.4%.

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Abstract

The invention relates to the field of medicine, in particular to a polypeptide having the ability to promote the expression of PER2 protein and to treat esophageal cancer. Its sequence is TQTDPLYIGGSALF, which is a brand-new sequence. This polypeptide can inhibit the proliferation of esophageal cancer cells TE1 and Eca109 cells in vitro and treat esophageal cancer. It has successfully increased the survival rate of mice in the in vivo tumor-bearing model experiment and has potential new drug development value. .

Description

technical field [0001] The present invention relates to PER2 protein agonist polypeptide 1 and application thereof, in particular to a polypeptide capable of promoting PER2 protein expression and treating esophageal cancer. Background technique [0002] In the treatment of esophageal cancer, surgery, radiotherapy, chemotherapy, and biological targeted therapy can be selected according to the location of the lesion and the stage of the disease. Esophageal cancer surgery often removes cancerous tissue and metastatic lymph nodes, and the tubular stomach replaces its function. However, surgery is difficult to completely remove cancerous tissue and subclinical lesions. The second is radiotherapy and chemotherapy, but both of these two treatment methods have obvious side effects on normal tissues and organs. Further improving the curative effect and reducing damage to normal tissues is a topic worthy of research. At present, biological targeted therapy for tumors is very promisi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08A61K38/10A61P35/00
Inventor 江晨
Owner 广州誉嘉生物科技有限公司
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