Zero-damage injection method of immune reaction allogenes of veneridae shellfish

Abstract

The invention discloses a zero-damage injection method of immune reaction allogenes of a veneridae shellfish. The method comprises the following steps that an injector with a syringe needle is prepared, a pathogen is sucked by the injector, the veneridae shellfish to be injected is prepared, an intersection point of the tail end of the outer ligament and the edge of the shell of the veneridae shellfish is found out, the intersection point serves as a collection point, the veneridae shellfish to be injected is stably held by one hand, the point of the syringe needle of the injector is aligned with the collection point by the other hand, the included angle between the point of the syringe needle and the tangent line of the edge of the shell is made to range from 30 degrees to 60 degrees, the point of the syringe needle is sent to the soft portion in the veneridae shellfish to be identified through the collection point, and the pathogen is injected into the veneridae shellfish. The injection method can be used for conducting a shellfish infection immunity test by injecting the pathogen into the shellfish on the premise that the shell of the shellfish is not damaged.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a non-injury input method for exogenous substances of the clam shellfish immune response. Background technique [0002] Among the shellfish cultured in my country, clam shellfish occupy an important breeding position. For example, the Philippine clam of the genus Clamidae is one of the four major cultured shellfish in my country and has high economic value. At the same time, the common clams in the market, such as clams, meretrix meretrix, shallow equilateral clams, and lamina clams, all belong to the shellfish of the clam family. Since clam shellfish are important edible shellfish, scientific researchers have conducted many studies on shellfish. In recent years, due to the gradual destruction of the environment, various pathogenic organisms have appeared in the shellfish, such as Parkinia in the Philippine clam, and the production of these pathogenic organ...

AI Technical Summary

Problems solved by technology

[0003] At present, in shellfish infection immunity tests, in order to inject various pathogens (bacteria, fungi, viruses, etc.) Special instruments or equipment are required, and the labor load is relatively large. At the same time, this destructive input method often causes the shellfish to produce a stress response, which in turn brings large errors to the experiment.

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