Manchurian wildginger total polysaccharide extract with cough-relieving activity, and extraction method and application thereof

An extraction method and technology for total polysaccharides, which are applied in the directions of organic active ingredients, medical preparations containing active ingredients, and drug combinations, can solve problems such as being unsuitable for industrialized large-scale production, low yield of total polysaccharides, and long extraction period, etc. Significant antitussive effect, high yield and content, and simple preparation method

Inactive Publication Date: 2014-09-10
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT) +1
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Our previous research found that Asarum volatile oil has obvious antitussive effect on the acute cough guinea pig model stimulated by citric acid, but the antitussive effect on the cough hypersensitive guinea pig model exposed to cigarettes is obviously not as good as the non-volatile component of Asarum total polysaccharide , and large doses have certain side effects
In addition, there are few research reports on the non-volatile components of Asarum. Bell amide Ⅰ, kaempferol-3-O-glucoside, etc. (Cai Shaoqing. Research on the non-volatile chemical components of Asarum. Beijing Medical University Journal. 1996,28(3):228-230; Shuai Lv. Chemical constituents of the underground part (Ⅱ). Journal of Shenyang Pharmaceutical University. 2010, 27(9): 707-710; Wei Qingchun. Isolation and identification of chemical constituents of Hancheng Asarum. Shi Zhen Guo Yi Guo Yao. 2010, 21 (3) :676-677)
The preparation method of asarum polysaccharide has been reported (Li Jingjing. S

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Manchurian wildginger total polysaccharide extract with cough-relieving activity, and extraction method and application thereof
  • Manchurian wildginger total polysaccharide extract with cough-relieving activity, and extraction method and application thereof
  • Manchurian wildginger total polysaccharide extract with cough-relieving activity, and extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0042] Example 1

[0043] Preparation of Asarum Total Polysaccharide Extract

[0044] 1kg of dried Asarum root and / or rhizome, crushed into coarse powder, sieved through an 80 mesh sieve, extracted with water 10 times the weight of the medicinal material, heated to reflux for 5 hours, the extract is filtered through 6 layers of gauze, concentrated and dried under reduced pressure To obtain the total extract of Asarum, heat and dissolve the total extract of Asarum to 1L with water, add 9 times the volume of absolute ethanol to 90% by volume, and stir while adding. After fully precipitation, place in a refrigerator at 4°C. Let stand overnight, pour the supernatant, precipitate under reduced pressure suction filtration, wash with the same amount of 90% volume percent ethanol twice, and dry routinely to obtain the Asarum total polysaccharide extract with a yield of 15wt%. The total polysaccharide content of Asarum was 68wt% and 60wt% determined by phenol-sulfuric acid method and anthr...

Example Embodiment

[0045] Example 2

[0046] Acute Toxicity Test of Asarum Total Polysaccharide Extract in Mice by Oral Gavage

[0047] 1 Experimental animals

[0048] SPF grade NIH mice, half male and half male, weighing 18-22g, provided by Guangdong Medical Experimental Animal Center, animal certificate number: SCXK (Guangdong) 2008-0002.

[0049] 2 Experimental method

[0050] With reference to "Experimental Methodology of Pharmacology" and "Experimental Methodology of Chinese Medicine Pharmacology", the formal Bliss method was used to investigate the acute toxicity of the total polysaccharide extract of Asarum polysaccharides administered orally to mice. First, use the maximum concentration, maximum dose, and maximum gavage volume to test. Before the test, without food and water for 12 hours, 10.0g / kg of Asarum total polysaccharide extract was given by gavage (equivalent to 24.4g / kg of crude drug, equivalent to 12 times of clinical dosage). The laboratory temperature is 25±2°C, once administered, o...

Example Embodiment

[0054] Example 3

[0055] Acute Antitussive Effect of Asarum Polysaccharide Extract on Guinea Pig Cough Caused by Citric Acid

[0056] 1 Experimental materials

[0057] Experimental animals: General Hartley guinea pigs, half male and half, weighing 250-350g, provided by Guangdong Medical Laboratory Animal Center, animal certificate number: SCXK (Guangdong) 2008-0002.

[0058] Reagents and instruments: citric acid monohydrate (Guangzhou Chemical Reagent Factory, batch number: 20121001-2), formulated into a 0.8M physiological saline solution; animal non-invasive Buxco lung function detection system (Buxco company in the United States); Aeroneb Pro nebulizer ( Aerogen (Ireland) Limited); Gavage needle (Guangdong Medical Laboratory Animal Center).

[0059] 2 Experimental method

[0060] The citric acid-induced acute cough model in guinea pigs was used to evaluate the efficacy. Take 128 Hartley guinea pigs and place them one by one in the animal non-invasive Buxco pulmonary function testing...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Half inhibitory concentrationaaaaaaaaaa
Login to view more

Abstract

The invention discloses a Manchurian wildginger total polysaccharide extract with cough-relieving activity and a preparation method and application thereof, belonging to the technical field of medicines. The Manchurian wildginger total polysaccharide extract is prepared by subjecting the root and/or tuber of Manchurian wildginger to extraction with a polar solvent, carrying out ethanol precipitation and then carrying out drying under reduced pressure, wherein the yield and the content of the total polysaccharide are 15 wt% and 60 to 80 wt%, respectively. Through a great number of pharmacodynamic tests, it is proved for the first time that the Manchurian wildginger total polysaccharide extract has a substantial cough inhibiting effect which is close to a treatment effect of codeine and shows a better effect on prolonging the incubation period of cough compared with codeine, and it is found out for the first time that the Manchurian wildginger total polysaccharide extract is capable of reducing sensitivity of cough and inhibiting airway inflammations, has good prevention and treatment effects on a variety of cough and can be used for preparation of drugs used for treating diseases related to cough. The Manchurian wildginger total polysaccharide extract has the advantages of definite activity, usage of original plants as experimental materials and low cost.

Description

technical field [0001] The invention belongs to the technical field of medicines, and more specifically relates to an extract of asarum total polysaccharides with antitussive activity, an extraction method and application thereof. Background technique [0002] Due to many factors such as work pressure and air pollution, the incidence of human respiratory diseases remains high, and cough is a common and frequently-occurring disease of the respiratory system, and it is a common complaint in respiratory clinics. Cough itself is a defensive reflex activity produced when the respiratory system is stimulated. It is a beneficial action and is sometimes seen in healthy people. In general, mild and infrequent coughs can be relieved naturally without the use of cough medicine. In addition, the impact of cough on human health is also dialectical, and it is also a pathological response. Long-term frequent or too intense cough will not only increase the suffering of patients, affect res...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K31/715A61P11/14
CPCA61K36/268A61K2236/00A61K31/715A61P11/14A61K2236/30
Inventor 赖克方刘晓东钟伯年钟山聂怡初
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap