Sex identification method for veneridae shellfish parents

An identification method, the technology of the clam family, which is applied in the direction of biochemical equipment and methods, and the determination/inspection of microorganisms, can solve the problems of unfavorable identification of individual survival, increase of test cost, cumbersome experimental process, etc., and achieve a simple and easy identification process , scientific research is of great significance, and the effect of broad market prospects

Active Publication Date: 2014-09-10
DALIAN OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the reference file is universally applicable to unrecognized dioecious molluscs, it requires special equipment (miniature electric drills), which increases the cost of the test, and the whole

Method used

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  • Sex identification method for veneridae shellfish parents
  • Sex identification method for veneridae shellfish parents
  • Sex identification method for veneridae shellfish parents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] In 2013, in the Engineering Technology Research Center of Shellfish Breeding in Liaoning Province, the sex of the equilateral clam was identified by the method disclosed in the present invention. Such as figure 1 shown.

[0020] First prepare a disposable sterile syringe with a volume of 1mL; prepare an equilateral clam to be identified, and determine the point where the end of the external ligament of the equilateral clam meets the edge of the shell. This point is the collection point. Hold it firmly with your left hand For equilateral clams, aim the needle tip of the syringe at the collection point with the right hand, adjust the tangent angle between the needle tip and the edge of the shell to be 35 degrees, send the needle tip of the syringe into the soft body of the equilateral clam, and let the needle tip enter according to the size of the equilateral clam. The position of the gonads of the equilateral shallow clam, sucking samples;

[0021] Put the sample taken...

Embodiment 2

[0024] In 2013, at the Engineering Technology Research Center of Shellfish Breeding in Liaoning Province, the clam sex was identified by the method disclosed in the present invention. Such as figure 2 shown.

[0025] First, prepare an ordinary syringe with a volume of 1mL, and sterilize it; prepare the clam to be identified, and determine the point where the end of the outer ligament of the clam meets the edge of the shell. This point is the collection point. Hold the clam firmly with your left hand, and Aim the needle point of the syringe at the collection point, adjust the tangent angle between the needle point and the edge of the shell to be 55 degrees, send the needle point of the syringe into the soft body of the clam, let the needle point enter the gonad of the clam according to the size of the clam, and draw the sample;

[0026] Put the sample taken out on a glass slide that has been dripped with seawater beforehand, and then observe the shape of the sample under a mi...

Embodiment 3

[0029] In 2013, at the Liaoning Provincial Shellfish Breeding Engineering Technology Research Center, the sex of the clam was identified by the method disclosed in the present invention. Such as image 3 shown.

[0030] First, prepare a disposable sterile syringe with a volume of 1mL; prepare the clam to be identified, and determine the point where the end of the outer ligament of the clam meets the edge of the shell. This point is the collection point. Hold the clam firmly with your left hand. For clams, point the needle tip of the syringe at the collection point with the right hand, adjust the tangent angle between the needle tip and the edge of the shell to be 45 degrees, send the needle tip of the syringe into the soft body of the clam, and let the needle tip enter the purple clam according to the size of the clam. The location of the gonads of the clam, taking samples;

[0031] Put the sample taken out on a glass slide that has been dripped with seawater beforehand, and...

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Abstract

A sex identification method for veneridae shellfish parents comprises the following steps: preparing a syringe with a syringe needle, preparing a to-be indentified veneridae shellfish, finding the intersection point of the lateral ligament end and the shell edge and taking the point as an acquisition point, stably handling the to-be indentified veneridae shellfish by using one hand, and using the other hand to align the syringe needle of the syringe with the acquisition point, and enabling the syringe needle to have an angle of 30-60 degree with the tangent of the shell edge, penetrating the syringe needle into the sexual gland position of the to-be indentified veneridae shellfish through the acquisition position, extracting a sample, putting the sample on slide glass with seawater, microscopically observing the form of the sample, if the sample is divergent in seawater and has circular or pear-shaped ova, identifying the indentified veneridae shellfish is female, if the sample is in a smog state in seawater and has polliwog-shaped sperms, identifying the indentified veneridae shellfish is male, and if the sample comprises circular or pear-shaped ova and polliwog-shaped sperms, identifying the indentified veneridae shellfish is hermaphroditic.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a method for identifying the sex of the parent of the clam shellfish. Background technique [0002] Among the shellfish cultured in my country, clam shellfish occupy an important breeding position. For example, the Philippine clam of the genus Clamidae is one of the four major cultured shellfish in my country and has high economic value. Common purple clams, meretrix meretrix, equilateral shallow clams, and thin clams that are common in the market belong to the clams of the clam family. Most clams of the clam family are dioecious, and a few individuals appear hermaphroditic. In dioecious shellfish, it is difficult to identify sex by appearance because of hermaphroditism, that is, no difference in secondary sex characteristics. Moreover, there is a sex reversal phenomenon in shellfish, dioecious can be gender-exchanged, and hermaphrodites can also become di...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 闫喜武杨凤胥贤赵力强聂鸿涛张跃环张兴志
Owner DALIAN OCEAN UNIV
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