Hydrodynamic focusing apparatus used for diffraction imaging flow cytometer

Abstract

The invention provides a hydrodynamic focusing apparatus used for a diffraction imaging flow cytometer. The hydrodynamic focusing apparatus comprises a sheath flow tube, a nuclear flow tube and a fluid chamber made of an optically transparent material, wherein the upper part of the sheath flow tube is located out of the upper port of the fluid chamber, the lower part of the sheath flow tube is embedded into the upper port of the fluid chamber, the lower part of the nuclear flow tube axially penetrates the center of the sheath flow tube from the upper port of the sheath flow tube and is located in the fluid chamber, the upper part of the nuclear flow tube is located out of the upper port of the sheath flow tube, and the lower port of the fluid chamber is provided with a liquid discharging pipe. The cross section of a liquid flow channel in the fluid chamber is a polygon, the outer wall of each edge is a plane, and the fluid chamber is prepared from the optically transparent material through bonding or sintering. According to the invention, processing of the sheath flow tube and the nuclear flow tube is simple; the hydrodynamic focusing apparatus can realize hydrodynamic focusing of nuclear flow in a great flow velocity adjusting range and obtain a stable layer fluid with accurately controllable nuclear flow position and flow velocity, so a high-contrast diffraction image of a to-be-measured particle can be obtained eventually.

Description

technical field [0001] The invention relates to a hydrodynamic focusing device for a diffraction imaging flow cytometer. In particular, it relates to a hydrodynamic focusing device for a diffraction imaging flow cytometer that can ensure stable laminar flow with precisely positioned nuclear flow at low flow rates and realize hydrodynamic focusing on the nuclear flow. Background technique [0002] Since the 1960s, in-depth optical measurement studies have been carried out on particles represented by cells in the state of rapid flow in the carrier fluid laminar flow. On the basis of these researches, flow cytometer technology has been formed, which is an instrument that can quickly measure and analyze a large number of single cells, integrating the research achievements of hydrodynamics, laser technology, photoelectric measurement and data processing. The flow cytometer uses concentric liquid pipes and fluid pressure differences to form a laminar flow consisting of sample flo...

AI Technical Summary

Problems solved by technology

In the incoherent imaging flow cytometer, the fluorescence image is an incoherent image due to the change of the fluorescence wavelength relative to the wavelength of the excitation beam, and the bright field or dark field image is generally obtained under the condition of incoherent white light illumination. It is also an incoherent image, which cannot analyze the three-dimensional structural characteristics of the particles to be measured

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