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A method to prepare Luo Hanson V from Luo Han Guo

A technology of mogroside and Luo Han Guo, which is applied in the field of preparing mogroside V, can solve the problems of high production cost, large energy consumption, and deepening of the color of the extract, and achieve the effects of avoiding damage, high yield, and short time

Active Publication Date: 2015-12-09
GUILIN SHILI SCI & TECH COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are certain defects in the Luo Han Guo extract obtained by the above method: the obtained extract has a deep color and has a strong taste of traditional Chinese medicine and a strong after-bitter taste, the content of active ingredients is low, and the color of the extract is deepened, which is not conducive to the follow-up. Disadvantages of decolorization and purification treatment, large energy consumption and high production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for preparing mogroside V from Luo Han Guo, specifically comprising the steps:

[0020] 1) Raw material treatment: take 100kg of fresh Luo Han Guo, wash and crush;

[0021] 2) High pressure treatment: put the broken Luo Han Guo into a high pressure tank, add 100kg of pure water, and pressurize to 4kg / cm 2 , make the pressure uniform, keep it for 15min, return to normal pressure, and get about 200kg of paste slurry;

[0022] 3) Supercritical CO 2 Fluid extraction: place the high-pressure-treated monk fruit paste slurry in an extraction kettle and extract it with supercritical CO. 2 The fluid is an extraction solvent, and an ethanol solution with a volume concentration of 60% is used as an entrainer to carry out supercritical extraction. The mass ratio of the paste slurry to the extraction kettle is 1:4, and the volume ratio of the ethanol solution to the extraction kettle is 1 : 2; the extraction temperature was 60°C, the extraction pressure was 15Mpa, and th...

Embodiment 2

[0025] A method for preparing mogroside V from Luo Han Guo, specifically comprising the steps:

[0026] 1) Raw material treatment: take 100kg of fresh Luo Han Guo, wash and crush;

[0027] 2) High pressure treatment: put the broken Luo Han Guo into a high pressure tank, add 100kg of pure water, and pressurize to 4kg / cm 2 , make the pressure uniform, keep it for 10min, return to normal pressure, and get about 200kg of paste slurry;

[0028] 3) Supercritical CO 2 Fluid extraction: place the high-pressure-treated monk fruit paste slurry in an extraction kettle and extract it with supercritical CO. 2 The fluid is an extraction solvent, and the ethanol solution with a volume concentration of 50% is used as an entrainer to carry out supercritical extraction, the mass ratio of the paste slurry to the volume of the extraction kettle is 1:4, and the volume ratio of the ethanol solution to the extraction kettle is 1 : 2; the extraction temperature was 50°C, the extraction pressure wa...

Embodiment 3

[0031] A method for preparing mogroside V from Luo Han Guo, specifically comprising the steps:

[0032] 1) Raw material treatment: take 100kg of fresh Luo Han Guo, wash and crush;

[0033] 2) High pressure treatment: put the broken Luo Han Guo into a high pressure tank, add 100kg of pure water, and pressurize to 4kg / cm 2 , make the pressure uniform, keep it for 12min, return to normal pressure, and get about 200kg of paste slurry;

[0034] 3) Supercritical CO 2 Fluid extraction: place the high-pressure-treated monk fruit paste slurry in an extraction kettle and extract it with supercritical CO. 2 The fluid is the extraction solvent, and the ethanol solution with a volume concentration of 55% is used as an entrainer to carry out supercritical extraction. The mass ratio of the paste slurry to the volume of the extraction kettle is 1:4, and the volume ratio of the ethanol solution to the extraction kettle is 1 : 2; the extraction temperature was 55°C, the extraction pressure w...

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Abstract

The invention discloses a method for preparing mogroside V from siraitia grosvenori. The method comprises the following steps of carrying out raw material treatment, high pressure treatment and supercritical CO2 fluid extraction, and collecting an extracting solution obtained after extracting; centrifuging, and carrying out HP-20ss resin column chromatography on supernatant; then, eluting by using a 50-60% ethanol solution, concentrating, and carrying out spray drying to obtain mogroside V. The method has the advantages of high content, simple process, low production cost and the like, and is suitable for production.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for preparing mogroside V from Luo Han Guo. Background technique [0002] Luo Han Guo, known as the "fairy fruit", is a unique and precious Cucurbitaceae plant in my country, and is known as a good medicine and a good fruit. Luo Han Guo belongs to the Cucurbitaceae plant, which is a multi-purpose medicinal and health care fruit. It is a unique medicinal plant in the world. It was recorded in the Chinese Pharmacopoeia in 1977 as a traditional Chinese medicine. One of the traditional Chinese medicines for food, according to books such as "The Dictionary of Traditional Chinese Medicine", Luo Han Guo is extremely sweet, cool in nature, non-toxic, enters the spleen and lung, and has the functions of clearing the lung and relieving cough, moistening the bowels, clearing heat and detoxifying, removing phlegm and nourishing blood, and benefiting the liver. ,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J17/00
CPCY02P20/544Y02P20/54
Inventor 何伟平
Owner GUILIN SHILI SCI & TECH COMPANY