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Method of scanning along a continuous scanning trajectory using a scanner system

A technology of scanning track and scanner, which is applied in the field of scanning along continuous scanning track by using a scanner system

Active Publication Date: 2017-04-26
FEMTONICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] One of the problems associated with the conventional RAMP mode is that measurements cannot be made during the switching time because the discrete change in frequency causes the focus to spread out in space, whereby multiphoton excitation no longer occurs

Method used

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  • Method of scanning along a continuous scanning trajectory using a scanner system
  • Method of scanning along a continuous scanning trajectory using a scanner system
  • Method of scanning along a continuous scanning trajectory using a scanner system

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example 1

[0066] In an exemplary setup, according to equation a 1x =b 1x t+c 1x and a 2x =b 2x t controls a 1x and a 2x .

[0067] In this case:

[0068]

[0069] Furthermore, employing a scanning system 100 in which the deflectors 12, 12' are identical: K 1x = K 2x = K x ,therefore:

[0070]

[0071]

[0072] Using these values, the frequency in a given deflector pair 10, for example x is:

[0073] f 1x = f 10x +b 1x t 2 +c 1x t and f 2x = f 20x +b 2x t 2

[0074] Using these, the x-coordinate can be determined:

[0075] x=f obj K x m x ((b 1x -b 2x )t 2 +c 1x t+(f 10x -f 20x )).

[0076] The above considerations can be similarly applied to control y-direction scanning:

[0077] a 1y =b 1y t+c 1y and a 2y =b 2y t and K 1y =K 2y =K y

[0078] From which the y-coordinate can be determined in the same way:

[0079] y=f obj K y m y ((b 1y -b 2y )t 2 +c 1y t+(f 10y -f 20y )).

[0080] Use settings for z(z x =z y and v zx =v zy )...

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Abstract

The invention relates to a method of scanning along a continuous scanning trajectory with a scanner system (100) comprising means for deflections generated by a continuous lens system (200) defining an optical axis (z) in the x-z plane The first pair of acousto-optic deflectors (10) of the focus of the electromagnetic beam and the second pair of acousto-optic deflectors (20) for deflecting the focus in the y-z plane perpendicular to the x-z plane substantially in the deflectors (12, 12') of the first pair (10) and in the deflectors (22, 22') of the second pair (20) of the deflectors (22, 22') continuously varying over time, so that The focal point moves continuously along the scanning trajectory.

Description

technical field [0001] The invention relates to a method of scanning along a continuous scanning trajectory with a scanner system comprising a first A pair of acousto-optic deflectors and a second pair of acousto-optic deflectors for deflecting the focal point in the y-z plane substantially perpendicular to the x-z plane. Background technique [0002] Three-dimensional (3D) laser scanning technology is important in performing measurements (including scanning, imaging, detection, excitation, etc.) on biological samples. [0003] The prior art typically employs two-photon laser scanning microscopy, which uses a laser of lower photon energy, where two photons are required to excite a fluorophore in a quantum event, resulting in the emission of a fluorescent photon, which subsequently detected by the detector. The probability of near-simultaneous absorption of two photons is extremely low, requiring a high flux of excitation photons, so two-photon excitation actually occurs on...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02F1/33G02B21/00G01N21/64
CPCG02F1/33G02F2201/16G02B26/101G02F1/332G02F1/291G02B21/004
Inventor B.罗萨G.卡托纳M.维雷斯P.马克G.萨莱A.卡斯扎斯B.乔维尼P.马特亚斯
Owner FEMTONICS