Rapid Propagation Technology of Ashitaba Stem Tissue Culture

Abstract

The invention discloses a rapid propagation technology of Ashitaba stem segment tissue culture. The stem segment of Ashitaba is selected, rinsed with running water, soaked in 75% alcohol solution, rinsed with sterile water, put into 0.1% mercury liter solution for disinfection, and then reused Rinse with sterile water; cut into sections; plant in callus induction medium and cultivate for 20-30 days to grow callus mass at the incision of stem segment; then cut callus mass into pieces and transfer to callus proliferation culture Culture on the substrate for 20-30 days to proliferate callus; cut the proliferated callus into pieces, transfer to the clustering bud induction medium and cultivate for 30 days to differentiate and produce clustering shoots; the callus at the base of clustering shoots is excised, Divide into individual cluster buds, inoculate the buds in the rooting medium and cultivate them for 20 days to obtain regenerated plant seedlings; one week after acclimating and training the seedlings, transplant them into sterilized coconut shell soil until the seedlings survive. The beneficial effect of the invention is that the ashitaba stem segments are used as propagation materials, and the cost is low.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and relates to a technique for rapid propagation of Ashitaba stem segment tissue culture. Background technique [0002] Ashitaba is used for both medicine and food, especially in terms of medicinal value, and is recognized by all walks of life as a new vegetable with great development potential. Our country introduced Ashitaba from Japan more than 20 years ago. At present, there are only a few domestic companies that have just introduced Ashitaba. The cultivation area is very small, and the raw materials have high prices in the international market and a large demand. In the production process, Ashitaba plants usually enter the reproductive period 1-2 years after planting, and die immediately after flowering and fruiting. The flowering period of Ashitaba lasts for a long time, the fruit setting rate is low, and the maturity of the seeds varies during harvesting, and the fruit is double...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a technique for rapid propagation of ashitaba stem segment tissue culture, which solves the problems of low germination rate of ashitaba seeds, insufficient raw materials and high prices in the market