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Near infrared rapid determination method of gallic acid content in terminalia chebula retz

A gallic acid, rapid determination technology, applied in the field of near-infrared measurement, to achieve the effect of strong stability, good reproducibility and high accuracy

Inactive Publication Date: 2014-11-05
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] In the prior art, there is no relevant report on the rapid determination of gallic acid content in Myrobalan by near-infrared spectroscopy

Method used

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Embodiment

[0039] Embodiment Adopt the following steps to realize the present invention:

[0040] 1. Collect the near-infrared spectrum of the sample:

[0041] (1) Sample pretreatment:

[0042] After the collected Myrobalan was dried, the pulp was peeled off, and the pulp was ground into a uniform, consistent powder of 40 mesh to prepare a sample set containing 89 samples.

[0043] (2) Determination of near-infrared spectroscopy of samples

[0044] The DA7200 near-infrared component analyzer produced by Sweden's Perten company is used, equipped with SimplicityTM spectrum acquisition software and The Unscrambler 9.8 (Norway Camo company) chemometric analysis software to collect sample near-infrared spectra: take 30g of myrobalan sample powder and put it into the near-infrared instrument In the self-contained sample cup, flatten it naturally, use the air as a reference, and collect the near-infrared spectrum after subtracting the background to obtain the near-infrared spectrum value of t...

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Abstract

The invention discloses a near infrared rapid determination method of gallic acid content in terminalia chebula retz. According to the method, a near infrared prediction model for the gallic acid content in the terminalia chebula retz can be established by combination of near infrared spectroscopy technology and partial least squares, the analysis determination of the gallic acid content in the terminalia chebula retz can be realized by use of the near infrared prediction model, compared with a conventional determination method of gallic acid, the method is a rapid and nondestructive determination method, and analysis and validation show that the near infrared prediction model obtained by the method is used in prediction of the gallic acid content in the terminalia chebula retz, has the advantages of strong stability, high accuracy and good repeatability; and the method provides the experimental basis for testing the quality of the terminalia chebula retz, and provides new ideas for quality identification of some other plants.

Description

technical field [0001] The invention relates to a near-infrared determination method, in particular to a near-infrared rapid determination method for gallic acid content in chebula. Background technique [0002] Myrobalan ( Terminalia chebula Retz ) is a very important medicinal plant. It is a very common Tibetan medicine and enjoys the reputation of "King of Tibetan Medicine". Myrobalan is often used in traditional medicine to cure a variety of ailments such as fever, cough, diarrhea, gastroenteritis, skin diseases, candidiasis, urinary tract infections and wound infections. The pharmacological components in Myrobalan are some hydrolyzable tannins (individual variation range is 20%-50%). Gallic acid (gallic acid, GA) is an important tannic acid in Myrobalan. Gallic acid is also known as "gallic acid" and "gallic acid", and its scientific name is "3,4,5-trihydroxybenzoic acid (C 7 h 6 o 5 )". [0003] Currently the most commonly used method for the determination of gal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/359G01N21/3563G01N30/02
Inventor 黄少伟刘纯鑫刘天颐吴征宇钟婷祝文娟
Owner SOUTH CHINA AGRI UNIV
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