Preparation method of airway mucus model
A mucus and airway technology, applied in the field of biomedicine, can solve the problems of not meeting the needs of experimental research and small sample capacity.
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example 1
[0008] 1. Add calcium chloride, sodium chloride, sodium hydrogen phosphate, and sodium dihydrogen phosphate to 10ml of deionized water to prepare a buffer.
[0009] 2. Add 0.1 g of DPPC to the phosphate buffer solution prepared above, and stir magnetically for 2 hours.
[0010] 3. Add 0.12g of HEC and 0.11g of BSA into the reaction solution and mix for 5 days with a vertical mixer.
[0011] 4. Add 1ml of GA solution cross-linking agent into the mixed solution to induce cross-linking reaction at 4°C.
[0012] 5. The solution after the cross-linking reaction was collected and freeze-dried.
example 2
[0014] 1. Add calcium chloride, sodium chloride, sodium hydrogen phosphate, and sodium dihydrogen phosphate to 10ml of deionized water to prepare a buffer.
[0015] 2. Add 0.1 g of DPPC to the phosphate buffer solution prepared above, and stir magnetically for 2 hours.
[0016] 3. Add 0.24g of HEC and 0.11g of BSA to the reaction solution and mix for 5 days with a vertical mixer.
[0017] 4. Add 1ml of GA solution cross-linking agent into the mixed solution to induce cross-linking reaction at 4°C.
[0018] 5. The solution after the cross-linking reaction was collected and freeze-dried.
example 3
[0020] 1. Add calcium chloride, sodium chloride, sodium hydrogen phosphate, and sodium dihydrogen phosphate to 10ml of deionized water to prepare a buffer.
[0021] 2. Add 0.1 g of DPPC to the phosphate buffer solution prepared above, and stir magnetically for 2 hours.
[0022] 3. Add 0.68g of HEC and 0.11g of BSA to the reaction solution and mix for 5 days with a vertical mixer.
[0023] 4. Add 1ml of GA solution cross-linking agent into the mixed solution to induce cross-linking reaction at 4°C.
[0024] 5. The solution after the cross-linking reaction was collected and freeze-dried.
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