Composition for detecting hot-spot mutation gene of lung cancer and application method thereof
A mutated gene and composition technology, which is applied in the field of compositions for detecting hot spot mutations in lung cancer driver genes, can solve the problems of poor detection specificity, inability to meet the needs of lung cancer detection, and low sensitivity.
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[0074] Example 1, screening of hotspot gene mutations in patients with lung cancer
[0075] 1. Experimental materials
[0076] 1. Collect tissue samples and matching plasma samples from 30 pathologically diagnosed lung cancer patients. After the plasma samples were collected, they were immediately stored in a -80°C refrigerator.
[0077] 2. The purity of all primers should reach electrophoresis grade (PAGE) or HPLC grade, free of impurities. Provide the quality inspection certificate of the synthetic product issued by the synthesis institution, such as PAGE electrophoresis results or HPLC analysis patterns, which prove that there should be obvious single-peak PAGE or HPLC purification patterns after purification by PAGE or HPLC, and the concentration is 10ng / μl for use.
[0078] 3. All reagents are purchased from regular manufacturers: DNA polymerase (Roche), 10×PCR Buffer (Roche), MgCl 2 (Roche), dNTP (TaKaRa), purified water.
[0079] 2. Main instruments: Roche Light Cyc...
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