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In-vitro culture system kit for improving sperm motility of human bodies and application method thereof

An in vitro culture system and sperm motility technology, which is applied in the field of culture system kits containing active recombinant proteins, can solve the problems of ineffective improvement of sperm motility, and achieve the goal of improving forward motility, pregnancy outcome, and fertilization rate Effect

Active Publication Date: 2014-12-10
杭州安体科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned culture medium mainly provides a buffer and a basic energy supply environment for sperm, and has no obvious effect on improving sperm motility

Method used

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  • In-vitro culture system kit for improving sperm motility of human bodies and application method thereof
  • In-vitro culture system kit for improving sperm motility of human bodies and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Sample source: three normal motility sperm samples (the standard is according to the fifth edition of WHO)

[0017] Sperm processing:

[0018] 1. Preheat 10mL of mHTF containing 10% SSS by volume (as a control group before culture), 2mL Isolate sperm separation solution and 10mL of the sperm culture system of the present invention.

[0019] 2. Place the semen collection cup in a 37°C water bath and take it out after liquefaction.

[0020] 3. Mix the preheated mHTF containing 10% SSS with the liquefied semen, slowly add it to the Isolate sperm separation medium, and centrifuge at 300g for 10 minutes.

[0021] 4. Discard the supernatant after centrifugation, remove the lower layer and separate the sperm for microscopic examination, record the sperm motility, and serve as the control group before culture.

[0022] 5. Mix the remaining sperm from step (4) with the sperm culture system of the present invention at a concentration of 10 million / mL, and place in a 37°C incuba...

Embodiment 2

[0026] Sample source: three sperm samples from patients with asthenozoospermia (the standard is according to the fifth edition of WHO)

[0027] Sperm processing:

[0028] 1. Preheat 10mL of mHTF containing 10% SSS by volume (as a control group before culture), 2mL of Isolate sperm separation solution and 10mL of the sperm culture system of the present invention.

[0029] 2. Place the semen collection cup in a 37°C water bath, and take it out after liquefaction.

[0030] 3. Mix the preheated mHTF with a volume fraction of 10% SSS and the liquefied semen, slowly add it to the Isolate separation solution, and centrifuge at 300g for 10 minutes.

[0031] 4. Discard the supernatant after centrifugation, remove the lower layer and separate the sperm for microscopic examination, record the sperm motility, and serve as the control group before culture.

[0032] 5. Mix the remaining sperm from step (4) with the sperm culture system of the present invention at a concentration of 10 mil...

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Abstract

The invention discloses an in-vitro culture system kit for improving the sperm motility of human bodies and an application method thereof. The kit comprises 9 mL of mHTF, 1 mL of serum substitute vcl recombinant proteins, and 45-55 mu g of acat2 recombinant proteins. The application method is implemented through mixing and balancing reagents in the kit for 2-3 hours at a temperature of 37 DEG C, so that 10 ml of a sperm culturing system is obtained; and after sperms separated from a sperm division fluid is resuspended by using the sperm culturing system according to a concentration of 10 millions / mL, gently and uniformly mixing, putting the obtained product in a thermostat at a temperature of 37 DEG C for 90-120 minutes. The forward motion activity of sperms resuspended by using the sperm culturing system in the invention can be significantly improved, and after the sperms are used in artificial insemination and in-vitro fertilization technologies, the pregnancy outcome can be improved, and the fertilization rate can be increased; and a new method is also provided for the treatment of patients with serious asthenospermia.

Description

technical field [0001] The invention relates to an in vitro culture system kit, in particular to a culture system kit containing active recombinant protein for improving sperm motility and its use method. Background technique [0002] In recent years, infertility patients have increased significantly, and male asthenospermia is one of the important reasons for male infertility. Asthenozoospermia refers to the proportion of forward motile sperm below 32% (a+b<32%), or the total sperm motility is below 40% (a+b+c<40%). For mild and moderate asthenozoospermia (a+b>10%), artificial insemination is often used clinically for treatment, but for severe asthenozoospermia (a+b<10%), in vitro fertilization or even single sperm follicle injection is used clinically and other technologies. Research has shown that better sperm motility is an important factor in the success of assisted reproductive technologies. [0003] Semen is composed of sperm and seminal plasma. Sperm o...

Claims

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Application Information

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IPC IPC(8): C12N5/076
Inventor 吴亦波陈玲王英男张帆金帆
Owner 杭州安体科技有限公司
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