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Method capable of improving animal sperm mobility in vitro and application thereof

A sperm motility and animal technology, applied in the field of microbiology, can solve the problems of improving sperm motility, achieve the effect of improving forward motility, increasing fertilization rate, and remarkable effect

Active Publication Date: 2019-05-28
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the above-mentioned culture solution can only provide a buffer and a basic energy supply environment for sperm, and basically has no effect on improving sperm motility. Therefore, it is particularly important to develop a method for improving sperm motility.

Method used

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  • Method capable of improving animal sperm mobility in vitro and application thereof
  • Method capable of improving animal sperm mobility in vitro and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Incubation and viability detection of weakly motile sperm

[0031] Specific steps are as follows:

[0032] (1) Take the composition raw material according to the formula of A-H group in Table 1;

[0033] (2) Mix the composition raw materials to obtain compositions A-H;

[0034] (3) Equilibrate the composition A-H at a temperature of 37° C. for 2 to 3 hours to obtain the balanced composition A-H;

[0035] (4) Preheat the sperm separation upper layer gradient medium (Isolate) at 37°C for 2 to 3 hours to obtain the preheated sperm separation upper layer gradient medium (Isolate);

[0036] (5) Put the semen collection cup with weak motility semen in a 37°C water bath for 0.5h to obtain liquefied semen;

[0037] (6) Mix the preheated sperm separation upper layer gradient medium (Isolate) with the liquefied semen, and centrifuge at 300g for 10 minutes to obtain the separated weakly motile sperm;

[0038] (7) Separate the weakly motile sperm according to 1×10...

Embodiment 2

[0046] Example 2: Incubation and Vitality Detection of Normal Motility Sperm

[0047] Specific steps are as follows:

[0048] (1) Take the composition raw material according to the formula of Group A in Table 1;

[0049] (2) Mix the composition raw materials to obtain composition A;

[0050] (3) Equilibrate the composition A at a temperature of 37° C. for 2 to 3 hours to obtain an equilibrated composition A;

[0051] (4) Preheat the sperm separation upper layer gradient medium (Isolate) at 37°C for 2 to 3 hours to obtain the preheated sperm separation upper layer gradient medium (Isolate);

[0052] (5) Place the semen collection cup with normal motility semen in a 37°C water bath for 0.5h to obtain liquefied semen;

[0053] (6) Mix the preheated sperm separation upper gradient medium (Isolate) with the liquefied semen, and centrifuge at 300g for 10 minutes to obtain normal motile sperm after separation;

[0054] (7) Separate the normal motility spermatozoa according to 1×1...

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Abstract

The invention discloses a method capable of improving the animal sperm mobility in vitro and application thereof, and belongs to the technical field of microorganisms. According to the method capableof improving the animal sperm mobility in vitro and the application thereof, a compound can significantly improve the forward movement mobility of animal sperms in vitro, then a pregnancy outcome is improved, and the fertilization rate is improved, so that the compound has large application prospects in the fields of artificial insemination and external fertilization separately; by placing normalperson sperms with normal mobility into the compound for incubation for 1.5-2 h, an average forward movement sperm ratio of the sperms with the normal mobility can be increased by about 15%; by placing asthenozoospermia patient sperms with weak mobility in the compound for incubation for 1.5-2 h, an average forward movement sperm ratio of the sperms with the weak mobility can be increased by about23%, so that the effects are significant.

Description

technical field [0001] The invention relates to a method capable of improving the motility of isolated animal sperm and its application, belonging to the technical field of microorganisms. Background technique [0002] According to the data of the World Health Organization, about 15% of couples of childbearing age in the world are facing the threat of infertility. The recent survey results in my country show that domestic infertility accounts for 10% of the number of married couples, and the incidence rate is on the rise. Among them, about half of human infertility patients are male infertility patients, and asthenospermia is one of the common causes of male infertility. [0003] Clinically, artificial insemination is often used to treat male infertility. However, artificial insemination often fails due to insufficient motility of male sperm. Therefore, improving sperm motility is an important condition for improving the success rate of assisted reproductive technology. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/076
Inventor 王英男杨勇袁艳毛健
Owner JIANGNAN UNIV
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