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A method for detecting pineapple stigma receptivity

A detection method and stigma technique, applied in the measurement of color/spectral characteristics, etc., to achieve the effect of accurate judgment

Active Publication Date: 2016-08-24
ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to propose an objective and quantitative pineapple method in view of the existing methods that rely on visual inspection to easily produce errors and the secretion of the stigma will form a protective layer on the stigma, preventing the enzyme at the stigma from contacting the reaction solution and thus affecting the detection results. Test method for stigma receptivity

Method used

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  • A method for detecting pineapple stigma receptivity
  • A method for detecting pineapple stigma receptivity
  • A method for detecting pineapple stigma receptivity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: (peroxidase test paper reaction liquid is to the mensuration of 6h pineapple stigma receptivity after anthesis)

[0030] Follow these steps:

[0031] (1) Preparation of reaction solution: Peroxtesmo KO peroxidase test paper produced in Germany and distilled water are impregnated with 1 piece: 1ml ratio to form reaction solution; each reaction solution is transferred to a 500ul centrifuge tube by 100ul / , for subsequent use;

[0032] (2) Preparation of pineapple stigma: cut off the fresh pineapple style 6h after flowering at 20-30mm from the stigma, and set aside;

[0033] (3) Determination of stigma absorbance values ​​A1 and A2: place the cut pineapple style with the stigma facing down, insert it upside down into a centrifuge tube until the stigma is immersed in the reaction solution and the incision at the base of the style is exposed on the liquid surface, and the stigma is in the reaction solution After immersion for 30s, both the immersion liquid part...

Embodiment 2

[0035] Embodiment 2: (benzidine-hydrogen peroxide reaction liquid is to the mensuration of stigma receptivity of 12 varieties of Yingge)

[0036] Follow these steps:

[0037] (1) Preparation of reaction solution: 1% benzidine and 3% hydrogen peroxide and water are prepared into benzidine-hydrogen peroxide reaction solution in a volume ratio of 4: 11: 22; by 150ul / each reaction solution is transferred to In 12 500ul centrifuge tubes, spare;

[0038] (2) Preparation of pineapple stigma: select 12 varieties from Pineapple genus Yingge, and each variety has 3 flowers that bloom at 7 o'clock in the morning on the same day, at 3 o'clock in the afternoon (that is, 8 hours after flowering) at a place 20-30mm away from the stigma Cut off the style, spare;

[0039] (3) Determination of stigma absorbance values ​​A1 and A2: Put 12 severed pineapple styles with the stigmas down, insert them upside down into 12 centrifuge tubes, until the stigmas are immersed in the reaction solution an...

Embodiment 3

[0043] Embodiment 3: (peroxidase test paper reaction liquid is to the mensuration of pineapple stigma receptivity in different florescences)

[0044] Follow these steps:

[0045] (1) Preparation of reaction solution: Peroxtesmo KO peroxidase test paper produced in Germany and distilled water are impregnated by 1: 1ml ratio to form a reaction solution; 200ul / each reaction solution is transferred to a 500ul centrifuge tube for subsequent use;

[0046](2) Preparation of pineapple stigma: cut one pineapple style 1h before flowering and 48h after flowering at a distance of 20-30mm from the stigma, and set aside;

[0047] (3) Determination of stigma absorbance values ​​A1 and A2: Put the cut pineapple styles with the stigmas facing down, and insert them upside down into the centrifuge tube until the stigmas are immersed in the reaction solution and the incision of the style base is exposed on the liquid surface, and the stigmas are in the reaction solution. After immersion in mediu...

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Abstract

The invention discloses a method for detecting pineapple stigma receptivity and belongs to the technical field of plant cross breeding. The method includes: (1) preparation of reaction solution; (2) preparation of pineapple stigma; (3) determination of stigma absorbance A1 and A2; (4) calculation of stigma receptivity A value and other steps. Compared with the traditional benzidine-hydrogen peroxide method, the present invention can quantify and determine the activity of stigma enzymes and the amount of stigma secretion through the change of light absorption value before and after the addition of surfactants, and can more accurately determine the stigma's ability to impart The strength of sex is more objective and accurate than the visual judgment of prior art.

Description

technical field [0001] The invention relates to the technical field of plant cross breeding, in particular to a method for detecting pineapple stigma receptivity. Background technique [0002] The receptivity detection of plant stigma is an important link in plant hybrid breeding, and the receptivity of stigma greatly affects the success rate of selfing and hybridization. Generally, before cross breeding, it is necessary to detect whether the plant stigma can be fertilized, the duration of fertilization and the fertile area, so as to select the female parent and judge the pollination timing and pollination area. [0003] The invention utilizes the presence of various enzymes and specific proteins on the surface of the plant stigma, and the activity and concentration of these enzymes and proteins will change with the change of the ability of the stigma to accept pollen. Changes in these enzyme activities can be detected by a variety of methods, thus reflecting changes in the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
Inventor 沈晓岚俞信英葛亚英王炜勇刘建新潘刚敏田丹青
Owner ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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