Growth medium and use thereof, and method for cultivating umbilical cord mesenchymal stem cells

A growth medium and basal medium technology, applied in animal cells, vertebrate cells, bone/connective tissue cells, etc., can solve problems such as poor expansion ability, and achieve the effect of improving expansion ability

Inactive Publication Date: 2014-12-24
THE THIRD MEDICAL CENT OF THE CHINESE PEOPLES LIBERATION ARMY GENERAL HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the defect of poor expansion ability in the existing method for culturing umbilical cord mesenchyma

Method used

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  • Growth medium and use thereof, and method for cultivating umbilical cord mesenchymal stem cells
  • Growth medium and use thereof, and method for cultivating umbilical cord mesenchymal stem cells

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preparation example Construction

[0029] Wherein, the preparation method of the growth medium of the present invention may include: mixing the basal medium with the additive.

[0030] The present invention also provides the use of the above-mentioned growth medium in culturing umbilical cord mesenchymal stem cells.

[0031] The present invention also provides a method for culturing umbilical cord mesenchymal stem cells, the method comprising: inoculating umbilical cord mesenchymal stem cells in the above-mentioned growth medium for culturing.

[0032] Among them, the culture temperature and CO 2The concentration can be a conventional choice in the field of umbilical cord mesenchymal stem cells; for example, the temperature of culture can be 36-38°C; the cultured CO 2 The concentration may be 4-6% by volume.

[0033] Wherein, the above-mentioned growth medium of the present invention can promote the rapid expansion of umbilical cord mesenchymal stem cells, correspondingly shortening the subculture period, so ...

preparation Embodiment 1

[0038] Fetal bovine serum (purchased from Gibco), bevacizumab (purchased from Roche), cetuximab (purchased from Merck), koji Tocilizumab (purchased from Roche) and guanosine-5-triphosphate trisodium salt (purchased from Sigma, hereinafter the same); the amount added makes the final concentration: fetal bovine serum 10% by volume, bevacizumab 0.08 ng / mL, cetuximab 0.06ng / mL, trastuzumab 0.15ng / mL and guanosine-5-triphosphate trisodium salt 1ng / mL; the growth medium of this preparation example was obtained.

preparation Embodiment 2

[0040] Fetal bovine serum (purchased from Gibco), bevacizumab (purchased from Roche), cetuximab (purchased from Merck), koji Tocilizumab (purchased from Roche) and guanosine-5-triphosphate trisodium salt; the amount added makes the final concentration: fetal bovine serum 10 volume%, bevacizumab 0.01ng / mL, cetuximab Antibody 0.1 ng / mL, trastuzumab 0.05 ng / mL and guanosine-5-triphosphate trisodium salt 300 μg / mL; the growth medium of this preparation example was obtained.

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Abstract

The invention discloses a growth medium for cultivating stem cells. The growth medium contains a basal culture medium and an additive, wherein the additive contains an antibody for resisting a human vascular endothelial growth factor, an antibody for resisting a human epidermal growth factor receptor 1, an antibody for resisting a human epidermal growth factor receptor 2 and vernine-5-sodium triphosphate. The invention also provides use of the growth medium in cultivation of umbilical cord mesenchymal stem cells, and also discloses a method for cultivating the umbilical cord mesenchymal stem cells. According to the method, the umbilical cord mesenchymal stem cells are inoculated and cultivated in the growth medium. Through the technical scheme, the in vitro amplification capability of the umbilical cord mesenchymal stem cells can be greatly improved.

Description

technical field [0001] The invention relates to the field of cell biology technology, in particular to a growth medium for culturing stem cells, the use of the growth medium and a method for cultivating umbilical cord mesenchymal stem cells. Background technique [0002] Stem cells are a type of undifferentiated or poorly differentiated cells with self-replication ability and multi-directional differentiation potential. Under certain conditions, it can differentiate into a variety of functional cells. According to the developmental potential of stem cells, they are divided into three categories: totipotent stem cells, pluripotent stem cells and unipotent stem cells. [0003] Mesenchymal stem cells, a kind of pluripotent stem cells, are important members of the stem cell family, derived from the mesoderm and ectoderm in the early stages of development. Mesenchymal stem cells (MSCs) have attracted increasing attention because of their multi-lineage differentiation potential,...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
Inventor 安沂华董健伸
Owner THE THIRD MEDICAL CENT OF THE CHINESE PEOPLES LIBERATION ARMY GENERAL HOSPITAL
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