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A kind of tyrosinase electrochemical biosensor and its application

A biosensor, tyrosinase technology, applied in the field of tyrosinase electrochemical biosensor, to achieve ultra-high selectivity, improve sensitivity and detection limit, and enhance the effect of electron transfer

Active Publication Date: 2017-02-08
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, there is no tyrosinase electrochemical biosensor based on mesoporous carbon-cobalt tetroxide-chitosan composite material for the detection of phenolic compounds at home and abroad.

Method used

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  • A kind of tyrosinase electrochemical biosensor and its application
  • A kind of tyrosinase electrochemical biosensor and its application
  • A kind of tyrosinase electrochemical biosensor and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1. Preparation of tyrosinase electrochemical biosensor

[0025] Glassy carbon electrode assembly steps:

[0026] (1) Polish the surface of the glassy carbon electrode with aluminum oxide powders with particle sizes of 1, 0.3, and 0.05 μm, and then repeatedly ultrasonically clean it in absolute ethanol and deionized water, and then dry the electrode surface with high-purity nitrogen stand-by. Put in 1mmolL -1 The cyclic voltammetry curve was scanned between -0.1 and +0.6V in the potassium ferricyanide / potassium ferrocyanide solution (molar ratio 1:1). The redox peak potential difference of the curve is less than 75mV, indicating that the redox reaction on the surface of the glassy carbon electrode is a completely reversible reaction, and the electrode polishing is in good condition, and the next experiment can be carried out.

[0027] (2) Construction of mesoporous carbon-cobalt tetroxide-tyrosinase composite material. The mesoporous carbon material was added...

Embodiment 2

[0030] Example 2. Standard solution for detection of phenolic compounds by tyrosinase electrochemical biosensor

[0031] Dissolve catechol, phenol, p-cresol, m-cresol and p-chlorophenol in water respectively to prepare a standard solution with a concentration of 1 mmol / L.

[0032]A certain volume (8μL) of catechol standard solution was continuously added dropwise (once every 40s) to the detection solution 8ml PBS of the three-electrode system composed of tyrosinase-modified glassy carbon electrodes, and the working potential was -0.1V Under the situation of constant potential scanning, the obtained current-time (I-t) relationship curve, because the concentration of catechol in the detection solution can be known at a fixed time point in the I-t curve, the correlation between the current intensity and the concentration of catechol can be obtained curve. Catechol is a natural substrate of tyrosinase. In the presence of oxygen, tyrosinase catalyzes catechol to generate o-quinone...

Embodiment 3

[0034] Example 3. Detection of interfering substances in tyrosinase electrochemical biosensors

[0035] Dissolve common interfering substances such as uric acid, glucose, and hydrogen peroxide in water, and prepare an aqueous solution with a certain concentration. When the concentration of catechol is 2 μM, add uric acid, glucose, and hydrogen peroxide aqueous solution dropwise to the detection solution. , scan the I-t curve, and obtain the electrical signal responses of the above three interfering substances. The results proved that the uric acid concentration of 40μM, the glucose concentration of 2mM and the hydrogen peroxide concentration of 0.1mM did not affect the detection of catechol by the tyrosinase electrochemical biosensor. Use the same detection process to detect inorganic salt ions (concentration is 2mM K + 、Na + , NO 3 - 、H 2 PO 4 - 、HPO 4 2- , Cl - 、Ac - Ions) and organic solvents (acetone, acetonitrile, ethanol) have carried out the interference test...

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Abstract

The invention discloses a tyrosinase electrochemical biosensor and its application. Firstly, tyrosinase is fixed in the pores of ordered mesoporous carbon, and then mixed with tricobalt tetroxide and chitosan to form GMC‑Co3O4‑Tyr‑Tyr‑ Chi composites were immobilized on the surface of glassy carbon electrodes to prepare tyrosinase electrochemical biosensors. The advantages of the present invention are: 1. High sensitivity to phenolic compounds; 2. Good selectivity; 3. Long-term stability; 4. The biosensor of the present invention is cheap in preparation, simple in sample pretreatment, fast in detection, and portable and suitable for On-site testing.

Description

technical field [0001] The invention belongs to biosensors, and relates to a biosensor based on ordered mesoporous carbon (GMC)-cobalt tetroxide (Co 3 o 4 )-chitosan (Chi) composite tyrosinase (Tyr) electrochemical biosensor for the detection of phenolic compounds in the aqueous environment. Background technique [0002] As the basic raw materials of chemical synthesis, phenolic compounds are widely used in industrial production, mainly from the production of chemicals such as pesticides, insecticides, plastics and pharmaceuticals. The discharge of these phenolic compounds into the water body will cause serious water environmental pollution, and has potential toxicity such as carcinogenicity, teratogenicity, and mutagenesis, and poses a huge threat to the ecological environment and human health. Phenolic compounds have attracted great attention from all over the world because of their high toxicity. In the 1970s, the United States listed 11 kinds of phenolic compounds amon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/48
Inventor 王雪卢宪波陈吉平
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI