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A kind of cultivation method of air pineapple tissue culture seedling

A cultivation method and technology of air pineapple, applied in the field of nursery stock breeding, can solve problems such as weak tissue culture seedlings of air pineapple, improper selection of cultivation containers, poor maintenance management, etc., to achieve the effect of ensuring excellent performance, high survival rate, and vigorous growth

Active Publication Date: 2016-08-17
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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Problems solved by technology

In the actual process of cultivating tissue cultured seedlings out of bottles, improper selection of cultivation containers and poor maintenance and management often occur, which eventually lead to the phenomenon of weak, thin, and even large-scale death of air pineapple tissue cultured seedlings. Therefore, the cultivation of tissue cultured seedlings Technical research is crucial

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  • A kind of cultivation method of air pineapple tissue culture seedling
  • A kind of cultivation method of air pineapple tissue culture seedling
  • A kind of cultivation method of air pineapple tissue culture seedling

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Embodiment Construction

[0038] The present invention will now be described in detail with reference to specific examples.

[0039] Test material: tissue-cultured seedlings of T. stricta variety through seed sowing tissue culture method, the height of the tissue-cultured seedlings is about 1.5-2cm, otherwise the tissue-cultured seedlings will easily dry up and die. Of course, the test materials can also be selected from T. bergeri (T. bergeri) and T. montana (T. montana) seed-propagated tissue culture seedlings among the cluster varieties.

[0040] And in the following examples, the 1 / 2MS nutrient solution used includes the components and contents as described in Table 1.

[0041] Table 1 Components and content of 1 / 2MS nutrient solution

[0042]

[0043]

[0044] The specific steps of cultivating air pineapple tissue culture seedlings are as follows:

[0045] (1) Seedling hardening: place the tissue cultured seedlings together with the container outside the cultivation room, open the mouth of...

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Abstract

The invention discloses a cultivation method of tissue culture seedlings of tillandsias. The method comprises the following steps that: (1) hardening-seedling, wherein tissue culture seedlings, with the height of 1.5-2cm, which are subjected to tissue culture by means of seed sowing and tissue culture are selected and subjected to hardening-seedling, and after 3-4 days, the tissue culture seedlings are out of bottles and transplanted; (2) transplantation, wherein culture mediums of the tissue culture seedlings which are out of the bottles in the step (1) are washed off and the tissue culture seedlings are transplanted; (3) maintenance management, wherein the tissue culture seedlings which are transplanted in the step (2) are revived for one month and subjected to maintenance management. The cultivation method of the tillandsias tissue culture seedlings has the advantages of high survival rate, high growth speed, vigorous growth and the like. The phenomena of withering, rotting and mildewed conditions are avoided; and furthermore, the high performance of tillandsias can be effectively guaranteed, and the increasing requirement on the tillandsias in the market can be effectively met.

Description

technical field [0001] The invention relates to a tissue culture rapid propagation method, in particular to a method for cultivating tissue cultured seedlings of Tillandsia pineapple, and belongs to the technical field of nursery stock breeding. Background technique [0002] Air pineapple (scientific name Tillandsia, English name Air plant) is a perennial herb of Bromeliaceae (Tillandsia), including nearly 550 varieties and 90 varieties, with a wide variety and different shapes. Air pineapple has a special function of purifying indoor air, but there are relatively few provenances of air pineapple in my country, and measures should be taken to speed up its reproduction. [0003] Since 2006, Jiangsu Agriculture and Forestry Vocational and Technical College has conducted research on the introduction, cultivation management, and rapid propagation of air pineapple. The use of tissue culture technology can greatly speed up the propagation of Tillandsia pineapple, including tissue...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G31/00
CPCA01G24/00A01G24/50A01G31/00
Inventor 郑凯丁久玲
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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