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Combination fertilizer synergist containing polyglutamic acid, phosphorus solubilizing bacteria and potassium solubilizing bacteria and its preparation method and application

A fertilizer synergist, polyglutamic acid technology, applied in the direction of microbial-based methods, biochemical equipment and methods, applications, etc., can solve problems such as agricultural product pollution, greenhouse effect, and soil compaction

Active Publication Date: 2020-06-09
轩凯生物科技(滁州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the excessive use of chemical fertilizers not only caused a lot of economic losses, but also brought many adverse consequences to our living environment, such as soil compaction, agricultural product pollution, water pollution and greenhouse effect.

Method used

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  • Combination fertilizer synergist containing polyglutamic acid, phosphorus solubilizing bacteria and potassium solubilizing bacteria and its preparation method and application
  • Combination fertilizer synergist containing polyglutamic acid, phosphorus solubilizing bacteria and potassium solubilizing bacteria and its preparation method and application
  • Combination fertilizer synergist containing polyglutamic acid, phosphorus solubilizing bacteria and potassium solubilizing bacteria and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Prepare the phosphate solubilizing bacteria agent of the present invention as follows:

[0083] Activate the phosphate-solubilizing bacteria Bacillus subtilis JT-1 stored on a slant at 4°C, inoculate it on the culture medium of an eggplant-shaped culture flask (ie, the activation medium), and cultivate it for 18-24 hours. Transfer the well-grown strains to the fermentation medium, culture at 30-35°C for 12-24 hours, and obtain the fermented liquid or the dried product of the fermented liquid. The number of bacteria in the bacterial agent can reach 1×10 10 -9×10 12 CFU / g.

[0084] The medium in the described eggplant-shaped culture bottle is LB medium, and the fermentation medium component is: glucose 10g / L, (NH 4 ) 2 SO 4 0.5g / L, NaCl 0.3g / L, MnSO 4 4H 2 O 0.03g / L, KH 2 PO 4 1g / L, pH 6.0-8.0.

[0085] The ventilation rate of sterile air is 0.6-0.8vvm in the fermentation culture process of above-mentioned production tank, and stirring speed is 180-200rpm.

Embodiment 2

[0086] Example 2: Determination of the phosphorus solubilizing ability of bacterial strains.

[0087] Add 100mL inorganic phosphorus liquid culture medium respectively in 500mL Erlenmeyer flask, medium composition is glucose 10g / L, (NH 4 ) 2 SO 4 1g / L, MgSO 4 ·7H 2 O 0.3g / L, NaCl 0.3g / L, FeSO 4 ·7H 2 O 0.03g / L, MnSO 4 4H 2 O0.03g / L, Ca 3 (PO 4 ) 2 5.0g / L. Then inoculate 1mL of bacterial suspension Bacillus subtilis JT-1, and at the same time do not inoculate the control (add 1mL sterile water to the medium), and repeat all treatments 3 times. Shake culture at 30°C and 150rpm for 7 days, centrifuge the culture solution at 6000rpm for 20min, measure the amount of phosphorus solution (water-soluble phosphorus content) by molybdenum antimony colorimetric method, and it is 682.43-725.28mg / L, and the phosphorus solution rate is 68.2% -72.5%. Phosphorus solubilizing bacteria JT-1 has a higher ability to solubilize phosphorus.

Embodiment 3

[0089] Prepare the potassium decomposing bacteria agent of the present invention as follows:

[0090] Bacillus subtilis JT-2 stored on a slant at 4°C was activated, inoculated on eggplant bottle medium (ie activation medium), and cultured for 24 hours. Pick a large number of strains from the eggplant bottle, inoculate them in the fermentation medium, and cultivate them at 30°C for 16 hours to obtain the fermentation liquid or the dry product of the fermentation liquid. The number of bacteria in the bacterial agent can reach 1×10 10 -9×10 12 CFU / g.

[0091] The medium in the described eggplant-shaped culture bottle is LB medium, and the fermentation medium component is: glucose 8-12g / L, (NH 4 ) 2 SO 4 0.05-1g / L, NaCl 0.1-1g / L, MnSO 4 4H 2 O 0.1-0.5g / L, KH 2 PO 4 0.5-2.0g / L, pH 6.0-8.0.

[0092] During the fermentation and cultivation process of the above-mentioned production tank, the ventilation rate of sterile air is 0.6-0.8vvm, the stirring speed is 180-200rpm, the ...

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Abstract

The invention discloses a combined fertilizer synergist containing polyglutamic acid, phosphate solubilizing bacteria and potassium bacteria. The combined fertilizer synergist comprises the following components in proportion: phosphate solubilizing bacteria, potassium bacteria and polyglutamic acid at the mass ratio of (0.1-1) to (0.1-1) to (0.001-0.1). The invention also discloses a preparation method and application of the fertilizer synergist. The phosphate solubilizing bacteria disclosed by the invention are capable of effectively releasing phosphate anions (PO4<3+>) in soil or fertilizer; the potassium bacteria are capable of effectively releasing potassium ions (K<+>) in the soil or fertilizer; after being compounded with the phosphate solubilizing bacteria and the potassium bacteria, the polyglutamic acid not only can provide a good growth environment for survival of the phosphate solubilizing bacteria and the potassium bacteria, but also can fully chelate nutrient elements released by the phosphate solubilizing bacteria and the potassium bacteria; and due to the synergistic effect, the effect of solubilizing phosphate and potassium is relatively outstanding, therefore the combined fertilizer synergist can be cooperatively used together with fertilizers, the utilization rate of the fertilizer is increased, and the combined fertilizer synergist can also be directly fertilized as a bacterial manure.

Description

technical field [0001] The invention relates to a combined fertilizer synergist containing polyglutamic acid, phosphorus solubilizing bacteria and potassium solubilizing bacteria, its preparation method and application, and belongs to the technical field of biological preparations. Background technique [0002] Chemical fertilizers are playing an increasingly important role in modern agricultural production. my country is a big country in the use of chemical fertilizers, with an annual consumption of more than 50 million tons, which has made important contributions to agricultural development and grain production. However, the excessive use of chemical fertilizers not only caused a lot of economic losses, but also brought many adverse consequences to our living environment, such as soil compaction, agricultural product pollution, water pollution and greenhouse effect. How to effectively improve the utilization rate of fertilizers and reduce the use of chemical fertilizers h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/00C05G3/80C05G3/40C05F11/08C12N1/20C12R1/125
CPCC05G3/00C05G3/80
Inventor 冯小海徐虹李莎许宗奇殷文锋
Owner 轩凯生物科技(滁州)有限公司
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