Two-dimensional fluid separation with controlled pressure
Patent Information
- Authority / Receiving Office
- CN · China
- Current Assignee / Owner
- AGILENT TECH INC
- Publication Date
- 2015-01-14
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Abstract
Description
technical field
[0001] The present invention relates to sample separation systems. Background technique
[0002] In liquid chromatography, a fluid sample and eluent (liquid mobile phase) are pumped through conduits and a column where separation of the sample components occurs. The column may comprise materials capable of separating the different components of the fluid analyte. This packing material (so-called particles, which may include silica gel) can be filled into a cylindrical tube, which can be connected to other elements, eg samplers, detectors, via conduits.
[0003] The composition of the mobile phase can be adjusted by composing the mobile phase from different fluid components with various contributions, the so-called gradient mode. HPLC systems are often operated in a gradient mode in which, for example, the organic content is ramped over time for reversed phase chromatography, or the salt content is ramped over time for ion exchange chromatography. Especially...