Delta 8 desaturase new gene derived from pavlova viridis

Abstract

The invention discloses delta 8 desaturase new gene derived from pavlova viridis. Polyunsaturated fatty acids, such as timnodonic acid EPA and docosahexaenoic acid DHA, are important nutrient substances which are necessary for a human body and cannot be synthesized by the human body per se. Aiming at microalgae, namely, pavlova viridis, which can synthesize EPA and DHA naturally, the application adopts conservative district sequence amplification combined with RACE to clone so as to obtain a new delta 8 fatty acid desaturase gene. Due to the obtaining of the gene, basis is provided for further knowing and utilizing an EPA and DHA biosynthesis process which has extremely great practical significance.

Description

technical field

[0001] The application belongs to the technical field of gene cloning and separation, and in particular relates to a desaturase gene related to the synthesis of polyunsaturated fatty acids derived from microalgae and its separation method. Background technique

[0002] Polyunsaturated fatty acids are essential nutrients for the human body, and microalgae are an important biological resource because they are rich in polyunsaturated fatty acids. Pavlova viridis is rich in EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid), and is a good experimental material for identifying and cloning polyunsaturated fatty acid-related synthesis genes.

[0003] The full name of RACE is rapid-amplification of cDNA ends, which is a commonly used technology for rapid cloning of cDNA ends by PCR. In this experiment, RACE technology was used to obtain the full-length sequence of the target mRNA fragment.

[0004] In the biosynthetic pathway of EPA and DHA in microalgae, ...

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