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A method for identifying inbred lines of Wuzhishan miniature pigs using 145 SNPs

A technology for miniature pigs and inbred lines, which is applied in the fields of biochemical equipment and methods, and the determination/inspection of microorganisms, which can solve the problems of non-inbred line establishment standards and identification methods.

Active Publication Date: 2016-07-20
GRAND LIFE SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since there is no report on the successful breeding of inbred pigs in the world, there is no standard and identification method for the establishment of inbred pigs

Method used

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  • A method for identifying inbred lines of Wuzhishan miniature pigs using 145 SNPs
  • A method for identifying inbred lines of Wuzhishan miniature pigs using 145 SNPs
  • A method for identifying inbred lines of Wuzhishan miniature pigs using 145 SNPs

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Embodiment 1, establishment of method

[0044] When the inventors of the present invention conducted genome research on Wuzhishan minipig inbred lines and non-Wuzhishan minipig inbred lines, they found that there were 145 SNP sites, and according to their genotypes, Wuzhishan minipig inbred lines and non-Wuzhishan minipig inbred lines could be identified. Miniature pig inbred strain. The genotypes of the 145 SNP loci of the Wuzhishan minipig inbred line were all homozygous (the genotypes described in Table 1). The 145 SNP loci of non-Wuzhishan minipig inbred lines were all heterozygous.

[0045]Among the 145 SNP loci, 12 are located on the first segment of chromosome 1, 16 are located on the 15th segment of chromosome 1, 14 are located on the 50th segment of chromosome 1, and 16 are located on the 78th segment of chromosome 1 , 15 on segment 2 of chromosome 2, 14 on segment 12 of chromosome 2, 13 on segment 19 of chromosome 2, 20 on segment 5 of chromosome 18, 13 on s...

Embodiment 2

[0061] Example 2. Using 145 SNPs to Identify Wuzhishan Miniature Pig Inbred Lines and Non-Wuzhishan Miniature Pig Inbred Lines

[0062] 1. Take experimental animals, take blood from the ear vein, and extract genomic DNA from the blood sample.

[0063] 2. Take the genomic DNA obtained in step 1, and hybridize with the nucleic acid chip immobilized with 145 probes (the 145 probes are respectively the single-stranded DNA molecules shown in sequence 1 to sequence 145 in the sequence listing).

[0064] 3. After step 2 is completed, each point in the nucleic acid chip is extended to obtain the genotypes of the 145 SNP sites in the genomic DNA.

[0065] 4. Take the genomic DNA obtained in step 1, perform whole genome sequencing, and obtain the genotypes of 145 SNP sites in the genomic DNA.

[0066] The result shows that the result of step 3 is exactly the same as the result of step 4.

[0067] The genotypes of the 145 SNP loci of the 48 experimental animals of the Wuzhishan minipig...

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Abstract

The invention discloses a method for identifying an inbred line of Gottingen strain. A method for assisting to identify whether a pig to be tested is of an inbred line of Gottingen strain comprises the following steps: detecting the genotype of the pig to be tested based on 145 SNP sites; if the genotype satisfies all the standards of (1) to (145), identifying that the pig to be tested is a candidate inbred line of Gottingen strain; if the genotype does not satisfy all the standards of (1) to (145), identifying that the pig to be tested is a candidate non-inbred line of Gottingen strain. The method for identifying the inbred line of Gottingen strain has an enormous value for the germplasm resource identification of the inbred line of Gottingen strain.

Description

technical field [0001] The invention relates to a method for identifying Wuzhishan miniature pig inbred lines by using 145 SNPs. Background technique [0002] From 1989 to 2003, "Wuzhishan Inbred Line Cultivation, Germplasm Characteristics and Development and Utilization" was included in the Ministry of Agriculture's "Seventh Five-Year, Eighth Five-Year, and Ninth Five-Year Plan" industry research projects, as well as the Ministry of Science and Technology's "863" and the National Natural Science Foundation of China. With the support of 7 subjects, such as , major projects, etc., the research work has made a breakthrough, that is: using 1 male and 1 female Wuzhishan pig (also known as Wuzhishan miniature pig) as the ancestor, continuously adopting "little pigs with mothers", "full compatriots" "Comprehensive measures such as mating, gradually overcome the adverse effects of inbreeding, which leads to extremely low reproductive survival rate and less than 20%, and form F 17 ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6827C12Q1/6837C12Q1/6888C12Q2600/124C12Q2600/156C12Q2565/607C12Q2565/518
Inventor 冯书堂牟玉莲韩建林李奎高倩
Owner GRAND LIFE SCI & TECH