Method for identifying and evaluating wheat scab expansion resistance

A technology for wheat head blight and head blight, applied in the field of identification and evaluation of plant disease resistance, can solve the problems of poor reproducibility of evaluation results, obvious environmental impact, etc., and achieve the effect of easy judgment, improved stability and consistency

Inactive Publication Date: 2015-01-28
YANGZHOU UNIV
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  • Abstract
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Problems solved by technology

[0003] The purpose of the present invention is to overcome the disadvantages of traditional single-flower instillation method in the evaluation of head blight resistance, such as the need for moisturizing measures, obvious environmental impact, and poor reproducibility of evaluation results, and provide a method that does not require moisturizing measures and can be used in field and greenhouse environments. Effective and Reliable Method for Evaluating Fusarium Scab Resistance Under Conditions

Method used

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Examples

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Embodiment Construction

[0021] The present invention uses the present invention to carry out scab inoculation and resistance evaluation on the known and recognized high head blight resistance variety NP154 and the high head blight variety NP516 under the condition of no moisturizing in the field. The resistance difference among the resistant materials was evaluated according to the time when the first diseased spikelet appeared after inoculation and the statistical diseased spikelet rate.

[0022] In order to better understand the implementation of this invention, we have listed the experimental material information of this invention: NP154 is a Chinese variety with high resistance to scab, which carries a scab resistance gene, and NP516 is a scab-susceptible variety introduced from the United States A diseased variety that does not carry any known scab resistance genes. Under the condition of single-flower instillation and moisturizing, the diseased spikelet rate of NP154 was between 0.05-0.16, whil...

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Abstract

The invention discloses a method for identifying and evaluating wheat scab expansion resistance. The method is mainly characterized in that after wheat ears, gibberella spore liquid is injected into internode stems below ears and not dropped into florets, and the wheat scab expansion resistance is comprehensively evaluated according to occurring time of a first disease spikelet and the rate of the disease spikelet in the 22-25th day after inoculation. The method has the greatest advantages that moisturizing measures are omitted after inoculation in a large field or greenhouse environment, and the method solves the problem that a traditional single flower dripping method needs the moisturizing measures such as bagging or spraying and water spraying for identification. Compared with the traditional single flower dripping method, the method is higher in inoculation and lower in cost, disease prevalence rate is decreased, phenotypic difference resisting and sensing capacity of varieties is maximized, and common technicians can more effectively judge the scab expansion resistance of wheat varieties.

Description

technical field [0001] The invention belongs to the technical field of identification and evaluation of plant disease resistance, in particular to a method for identifying and evaluating extended resistance to wheat head blight. Background technique [0002] At present, the mainstream method for the evaluation of scab extended resistance is the single flower drip method, that is, inject spore liquid to one of the florets at the ear of wheat at the flowering stage, mark the inoculation time, and then keep the humidity with a plastic bag for 72 hours tear or remove the plastic bag after inoculation; or use pre-installed spray or water spray equipment to spray or spray water regularly after inoculation to moisturize, and count the incidence rate 18-21 days after inoculation, that is, the proportion of diseased spikelets to total spikelets The percentage of the number was used to evaluate the disease resistance. The efficiency of plastic bagging is extremely low, and at the sam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00A01G7/06
CPCA01G7/06C12Q1/18
Inventor 李韬李磊郑飞董晶晶
Owner YANGZHOU UNIV
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