Device for performing freeze fracture on scanning electron microscope sample

Abstract

The invention relates to the technical field of pathology equipment and solves the technical problems that liquid nitrogen is not fully precooled, an operator is easily accidentally frozen, the knocking strength is non-uniform, the direction offsets and the natural section is bad in the process of performing freeze fracture on the conventional scanning electron microscope sample. With the adoption of the technical scheme, the device comprises a freeze fracture device main body and a master control table, wherein the freeze fracture device main body comprises a liquid nitrogen tank, a sample stage, fixing devices on two sides of the sample stage, a cutting knife, a cutting knife arm and a liquid nitrogen circulating device and a resetting device in the cutting knife arm, a knocking device, a knocking device arm and a resetting device in the knocking device arm; and the master control table comprises a fixation regulating device, a sample stage regulating device, a knocking strength regulating device, a liquid nitrogen circulation starting device and a knocking starting device. According to the technical scheme in the invention, due to the combined operation of the freeze fracture device main body and the master control table, the verticality and strength stability in the knocking direction are guaranteed, a natural section is easily formed, the device is easy and convenient to use, the manual labor is saved, low-temperature frostbite is avoided, and the manufacturing success rate is improved.

Description

technical field [0001] The application belongs to the technical field of pathological instruments, and in particular relates to a device for freezing and fracturing a scanning electron microscope sample. Background technique [0002] The freeze-fracture method is a sample preparation technique used for scanning electron microscopy to observe the microstructure of tissues and cells. By freezing and fracturing in liquid nitrogen, the tissue produces a natural cross-section for easy observation. At present, the commonly used method is to pre-cool the metal plate and the blade in liquid nitrogen in advance, place the fixed tissue specimen on the metal plate for quick freezing, hold the pre-cooled blade with a hemostat in one hand, and place the lower end of the blade on the metal plate. At the place where the tissue is to be broken, another hand-held mallet hits the upper end of the blade to quickly break the frozen tissue and produce a natural cross section. The freezing fractu...

AI Technical Summary

Problems solved by technology

In this method, both the metal plate and the blade need to be pre-cooled with liquid nitrogen, and the operation process needs to be fast, which may easily cause frostbite to the operator. In addition, at the moment of freezing fracture, it is difficult to grasp the verticality of the blade and the direction and strength of the mallet, which may easily lead to After the fracture, the tissue is fragmented, splashed, and lost, and it is not convenient to produce the natural section required for observation, and this operation needs two people to complete it at the same time, which is very inconvenient

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