Segmenting method of gecko brain atlas processed through hematoxylin-eosin (HE) staining

A brain atlas and gecko technology, applied in the field of image processing, can solve problems such as monotonous colors, difficult segmentation, over-segmentation, etc.

Inactive Publication Date: 2015-01-28
YANCHENG INST OF TECH
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  • Abstract
  • Description
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Problems solved by technology

At present, the usual research method is nuclear magnetic resonance, but its shortcomings are obvious. The amount of information provided by its images is not very rich, mainly due to the monotony of its color, especially for small tissues such as the gecko brain. The amount of information that can be provided is even more limited. In order to break through the limitations of the MRI method, the brain tissue is made into paraffin sections, and different tissues can be displayed in different colors through HE staining.
It is photographed by a microscope digital camera, and various tissues are mixed together in...

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  • Segmenting method of gecko brain atlas processed through hematoxylin-eosin (HE) staining
  • Segmenting method of gecko brain atlas processed through hematoxylin-eosin (HE) staining
  • Segmenting method of gecko brain atlas processed through hematoxylin-eosin (HE) staining

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Embodiment Construction

[0022] The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0023] Such as figure 1 As shown, the present invention designs a kind of segmentation method of the Gecko brain map through HE staining, comprises the following steps:

[0024] Step 001. Stain the paraffin sections of the gecko brain tissue with HE staining, specifically including the following steps: As a preferred technical solution of the present invention: the step 005 includes the following processing procedures: (1) xylene I: soaking 5 to 10min, (2) xylene II: soak for 5 to 10min, (3) absolute alcohol I: soak for 1 to 3min, (4) absolute alcohol II: soak for 1 to 3min, (5) 95% alcohol I: soak 1 to 3min, (6) 95% alcohol II: soak for 1 to 3min, (7) 80% alcohol I: soak for 1min, (8) distilled water: soak for 1min, (9) Hematoxylin semen staining: soak for 5 to 10min, ( 10) Wash away hematoxylin semen with running wat...

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Abstract

The invention relates to a segmenting method of a gecko brain atlas processed through hematoxylin-eosin (HE) staining. Aimed at color features of tissues on the brain atlas shot by a Microscope or a digital camera, the segmenting method based on an YCbCr space is provided. According to the segmenting method of the gecko brain atlas processed through the HE staining, firstly, the color of the atlas is converted from an RGB space into the YCbCr space, then the brain atlas is segmented according to distribution differences of components Cb and components Cr in different regions of the atlas, and finally the different tissues in the atlas are completely segmented. The segmenting method provides a more convenient condition for researches on the brain structures of geckos and corresponding biobots.

Description

technical field [0001] The invention relates to a segmentation method of an HE-stained gecko brain atlas, which belongs to the field of image processing. Background technique [0002] Since the 9 / 11 incident, all parts of the world have increased investment in anti-terrorism, especially the research on anti-terrorism robots. The progress in this area will further promote the progress of anti-terrorism technology. At present, the widely used mechanical robot has developed to a certain position, but its shortcomings are also obvious, that is, the energy it can carry is very limited, and it is easy to be found. In view of such drawbacks, another robot is currently being developed Quietly rising, that is, bio-robots. By inserting electrodes into a specific area of ​​the brain of the creature and discharging it, the creature can be controlled to move according to the specified command. It can supplement its own energy by looking for external food, and it is difficult to find. B...

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Application Information

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IPC IPC(8): G06T7/00
CPCG06T2207/10061G06T2207/30016
Inventor 袁铁军孟龙晖张秀丽
Owner YANCHENG INST OF TECH
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