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Microsatellite molecular marker related to growth character of megalobrama amblycephala and application of molecular marker

A technology of growth traits and molecular markers, applied in the field of microsatellite molecular markers and applications, can solve the problems of long breeding cycle, lack of pre-selection methods for target traits, and low efficiency, and achieve the effect of saving manpower and reducing experimental workload

Inactive Publication Date: 2015-02-04
HUAZHONG AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Conventional breeding is based on the phenotype of the trait instead of the genotype. There is no pre-selection method for the target trait, and the breeding cycle is long and the efficiency is low.
So far, there are no reports on molecular markers related to growth traits of bream

Method used

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  • Microsatellite molecular marker related to growth character of megalobrama amblycephala and application of molecular marker
  • Microsatellite molecular marker related to growth character of megalobrama amblycephala and application of molecular marker
  • Microsatellite molecular marker related to growth character of megalobrama amblycephala and application of molecular marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Screening of microsatellite markers related to growth traits of bream:

[0023] (1) Source of material

[0024] In June 2009, 42 female parents and 50 male parents from Liangzi Lake, Silt Lake and Poyang Lake were artificially bred, and the offspring were cultured in the same pond. In March 2011, the F1 generation of bream was randomly salvaged in the pond, numbered, body length, height and weight were measured, and the fin rays were cut and stored in 100% alcohol for later use. According to the body weight data, select the individuals whose body weight is greater than 0.4kg, which is recorded as the large individual group (Top), and each 96 individuals whose body weight is less than 0.28kg, is recorded as the small individual group (Below), and carry out microsatellite genotype analysis as experimental objects; At the same time, 60 individuals in each group were used as the size group for the preliminary screening of microsatellite loci related to the growth traits of...

Embodiment 2

[0044] Positive and negative effects of Mam166 and Mam116 genotypes:

[0045] Multiple comparisons were performed on the genotypes of the two loci, and the disadvantageous alleles at each microsatellite marker locus were deduced, so as to preliminarily judge whether the above genotypes had positive or negative effects on the traits.

[0046] The genotype of the site described in the present invention is based on the band size (bp) displayed after polyacrylamide gel electrophoresis and silver nitrate staining of the site PCR product read by Quantity One software. For example, at the site Mam116, the 121 / 121 genotype means that only one band of 121 bp is amplified by the primers; the 154 / 176 genotype means that two bands of 154 bp and 176 are amplified by the primers.

[0047] The multiple comparison analysis of the genotypes of the two sites found that in the site Mam116, a total of 11 genotypes were obtained, and there was no significant difference in body weight among the 121...

Embodiment 3

[0057] The application of microsatellite molecular markers related to the growth traits of bream in molecular assisted breeding of bream, the application process is as follows:

[0058] 1) From the analysis results of the two loci, respectively select the 3 positive effect genotypes with the largest average value and the 3 negative effect genotypes with the smallest average value as the selection criteria (if not enough), the results are shown in Table 4.

[0059] Table 4 Positive and negative effect genotypes of two loci

[0060]

[0061] 2) Genotype analysis was carried out in the 72 individuals of the F1 generation (Example 1) of the verification population, and individuals with positive or negative effect genotypes were selected for multiple comparison and significant difference analysis of growth traits. The results are shown in Table 5.

[0062] Table 5 Individual analysis of positive or negative effect genotypes in the validation population

[0063]

[0064]

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Abstract

The invention discloses a microsatellite molecular marker related to growth character of megalobrama amblycephala and application of the molecular marker. Through clustering separation method, association analysis on the molecular marker and character is realized, two molecular markers obviously related to growth characters are finally screened; a primer of Mam116 is as follows: F: 5'-CTATTTACAGTTTCATGCTTTCCTC-3' and R: 5'-ATCCCGTCCGCCGCTTACT-3'; a primer of Mam166 is as follows: F: 5'-GGTACTGTTTGTGCTGGGC-3' and R: 5'-CTGCTCACTCAACTTATTGTAGGTC-3'. According to the molecular marker, multiple comparisons on different genotype characters are carried out on the two markers, so as to determine inferior and superior alleles, and individuals containing positive genotypes are selected from megalobrama amblycephala character related breeding as breeding parents while individuals containing negative genotypes are avoided, so as to reduce the experimental workload and accelerate the breeding efficiency.

Description

technical field [0001] The invention relates to fish molecular marker-assisted breeding technology, in particular to microsatellite molecular markers related to growth traits of bream and their application. Background technique [0002] Megalobrama amblycephala Yih (1955), also known as Wuchang fish, belongs to Osteichthyes, Cypriniformes, Cyprinidae, and genus Bream. Its natural distribution is limited to a few lakes in the middle and lower reaches of the Yangtze River. Because of its delicious taste, wide appetite, fast growth and easy fishing, it is widely loved by consumers and farmers. Due to the widespread inbreeding phenomenon in the production of bluehead bream fingerlings, the germplasm degradation is exacerbated, and the growth rate of conventional seedlings is relatively slow. Although my country has selected and bred Pujiang No. 1, which has grown faster, the seedlings still cannot meet the market demand. It is still necessary to further strengthen the monitori...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 高泽霞王卫民陈柏湘刘肖莲赵鸿昊万世明罗伟
Owner HUAZHONG AGRI UNIV
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