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Method for in vitro intermediate propagation of skimmia reeuesiana

A technology of inocula and red star, which is applied in the field of in vitro rapid propagation of red star inoculation, can solve the problems of limited pedicel materials, no rooting culture test, and seedling breeding restrictions, and achieve the effect of simple and convenient material collection, strong root system, and easy germination

Inactive Publication Date: 2015-02-11
WUHAN INST OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the time limit for the flowering of Echinococcus chinensis, the use of flower stalks is limited, and there is no experiment on rooting culture in the experiment, so it is limited in the actual factory seedling cultivation

Method used

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  • Method for in vitro intermediate propagation of skimmia reeuesiana
  • Method for in vitro intermediate propagation of skimmia reeuesiana
  • Method for in vitro intermediate propagation of skimmia reeuesiana

Examples

Experimental program
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Effect test

Embodiment 1

[0037] A kind of method for in vitro rapid propagation of Rhododendron chinensis, the steps are:

[0038] 1. Medium preparation:

[0039] 1.1 Basic medium preparation:

[0040] The medium is MS, respectively take 100ml of macroelement mother liquor; 10ml of MS trace element mother liquor; 10ml of MS organic mother liquor; 10ml of MS iron salt; 10ml of inositol;

[0041] Table 1 MS medium mother solution formula

[0042]

[0043] 1.2 Growth hormone preparation:

[0044] Add the required cytokinin 6-BA (6-benzyladenine) \ auxin NAA (naphthalene acetic acid), IBA (indole butyric acid), GA 3 (Gibberellin) was dissolved in 0.1mol / L NaOH or ethanol, diluted with distilled water, and then prepared into 0.5mg / ml mother liquor, put into a volumetric flask, and stored in a refrigerator.

[0045] 1.3 Culture medium preparation:

[0046] All media were added with agar powder 7g / L, sucrose 30g / L and different concentrations of plant growth regulators, and the prepared media were ad...

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Abstract

The invention discloses a method for in vitro intermediate propagation of skimmia reeuesiana. The method comprises the following steps: (1) selecting and disinfecting an explant: by using a mature skimmia reeuesiana seed the explant, disinfecting by using 70% alcohol and then disinfecting by using 0.1% HgCl2 and washing by using sterile water; (2) seed germination: inoculating the disinfected seed into germination culture for culturing, wherein the seed germinates to enable cotyledon and euphylla to emerge; (3) calluses induction and proliferation: slicing a terminal bud with the euphylla, inoculating to a proliferation medium for subculture, wherein calluses are continuously proliferated; (4) rooting culture: inoculating a test-tube plantlet obtained by expanding propagation to a rooting medium and culturing the seedling to differentiate a white adventitious root; and (5) domestication and transplanting. The method is simple, convenient and feasible, great in propagation coefficient, low in production cost and short in period. The average propagation coefficient reaches 4.5, the rooting percentage is 90% and the survival rate of transplanting is 95%. The method is simple and convenient to operate and great in propagation coefficient and lays a foundation for industrial production of skimmia reeuesiana sprouts.

Description

technical field [0001] The invention relates to the field of plant biotechnology, and more specifically relates to a method for in vitro rapid propagation of the red star indica. Background technique [0002] Skimmia japonica Rubella (Skimmia japonica Rubella), also known as red jade pearl, belongs to the Rutaceae Rubella plant, and is one of the popular high-end ornamental flowers in recent years. The leaves of the taro are emerald green and bright, and the reddish-brown flower buds in early summer are exquisite and lovely, with a strong fragrance; in autumn and winter, the red stars are full of branches, bright and dripping, and the fruit-bearing period can last for more than half a year. It is an excellent flower for viewing leaves, flowers, and fruits. Species (Wu Difei, New Year's Eve Fruit Watching Jiahui a red star fungus taro. Chinese flower bonsai, 2007, (2): 5-5). [0003] The red star taro can be propagated by sowing and cuttings. If seeds are used for propagatio...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 李其友阳永学曾红霞肖康飞祝菊红程维舜张娜杨皓琼毕秋荣
Owner WUHAN INST OF AGRI SCI
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