Liquid chromatography detecting method for 2,4-methylimidazole and 2-acetyl-4-tetrahydroxyl-buthylimidazole in caramel pigment

A technology of methylimidazole and butylimidazole, which is applied in the inspection field of food safety inspection, to achieve the effect of accurate and reliable measurement results, effective separation and purification

Inactive Publication Date: 2015-02-18
JIANGSU PROVINCIAL CENT FOR DISEASE PREVENTION & CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the methods can only detect one or two substances in 2-MEI, 4-MeI or THI

Method used

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  • Liquid chromatography detecting method for 2,4-methylimidazole and 2-acetyl-4-tetrahydroxyl-buthylimidazole in caramel pigment
  • Liquid chromatography detecting method for 2,4-methylimidazole and 2-acetyl-4-tetrahydroxyl-buthylimidazole in caramel pigment
  • Liquid chromatography detecting method for 2,4-methylimidazole and 2-acetyl-4-tetrahydroxyl-buthylimidazole in caramel pigment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0049] (1) The preparation of the sample solution to be tested includes the following specific steps:

[0050] (a) Preparation of solid-phase extraction sample solution: Weigh 1 g of caramel pigment sample (accurate to 0.001 g), put it in a 10 mL volumetric flask, and add water to make up to the mark. Take 0.2 mL of the above sample solution, add 0.1 mL of hydrochloric acid solution (0.1 moL / L), add water to make up to 2 mL, mix well and wait for purification by solid phase extraction.

[0051] (b) Solid-phase extraction column activation: choose a mixed-type cation-exchange solid-phase extraction column with a matrix of polystyrene-divinylbenzene polymer, 60 mg, 3 mL, or equivalent. Activate with 2 mL of methanol followed by 2 mL of water before use.

[0052] (c) Solid-phase extraction: Add the sample solution prepared in (a) to the freshly activated small column in (b), control the flow rate to less than 0.5 mL / min, drain it, add 1 mL of water and 1 mL of methanol to rinse ...

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Abstract

The invention discloses a detecting method for byproducts 2-methylimidazole, 4-methylimidazole and 2-acetyl-4- tetrahydroxyl-buthylimidazole in caramel pigment by combining solid-phase extraction with high-efficiency liquid chromatography. The method comprises the following steps: 1. preparing a to-be-detected sample solution; 2. detecting a sample; and 3. analyzing results. The method disclosed by the invention has the characteristics of quick detection, high sensitivity, high degree of accuracy, low cost, strong universality, and the like.

Description

Technical field [0001] The present invention involves a detection method of 2-beamimidazole, 4-methylimramidazole, and 2-acetyl-4-acetobichimazole in a caramel pigment, which is a test method for food safety testing. Background technique [0002] Caramel pigment is a food additive, commonly known as sauce color, which is a thick reddish -brown colloidal substance, accounting for more than 90 %of the total sales of the entire food.It can be applied to soy sauce, vinegar, beer, pickles, canned food, candy, soup, roasted food, grilled smoke and cough syrup and other aspects.Two major categories of reactions occur during the formation of caramel pigment -the Marada response and caramelization reactions. Among them, the main caramel response occurs during the production process of ordinary caramel pigments.Traculose pigment production is mainly Marada's reaction.Studies have found that many chemicals that can produce many harmful to the human body during the reaction process, such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 荣维广马永建吉文亮
Owner JIANGSU PROVINCIAL CENT FOR DISEASE PREVENTION & CONTROL
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