Rapid high-throughput screening method for oil-recovery microorganisms

A high-throughput technology for oil production microorganisms, applied in biochemical equipment and methods, and microbial measurement/inspection, etc., can solve the problems of expensive instruments and reagents, and the inability to quickly screen functional oil production bacteria, and achieve efficient screening and easy promotion. Effect

Inactive Publication Date: 2015-02-25
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Commonly used rapid screening microbial techniques mainly include: spiral plate counting method, filter membrane method, paper method, and ATP method, electrochemical method, color change, flow cytometry, laser scanning technology, calorimetry and radiometric method, as well as There are microfluidic technologies based on gene chips, but most of these technologies for rapid detection of microorganisms are used in the field of food, drug and cosmetic inspection. In the detection of microorganisms in oil reservoirs with complex sample components, the application is still quite important. limitations
Although the application of modern molecular biology has greatly enriched the understanding of the genetic information of oil reservoir microorganisms, its biggest defect is that it cannot quickly screen cultivable functional oil production bacteria that can meet the requirements of field tests, and the required Instruments and reagents are relatively delicate and expensive, and operators need to undergo professional training and have solid basic knowledge of biology. It is difficult for general oilfield subordinate units to meet the matching conditions of hardware and software

Method used

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  • Rapid high-throughput screening method for oil-recovery microorganisms
  • Rapid high-throughput screening method for oil-recovery microorganisms
  • Rapid high-throughput screening method for oil-recovery microorganisms

Examples

Experimental program
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Embodiment 1

[0037] The method for rapid high-throughput screening of oil recovery microorganisms in this embodiment comprises the following steps:

[0038] (1) Open the sampling port to release the near-end oil sample or water sample, (after a period of time) bring the pre-sterilized sampling container close to the sampling port, and take a sufficient amount of oil sample or water sample;

[0039] (2) After all samples are collected, they are directly sealed and labeled, and sealed with plastic bags (to ensure minimum external pollution);

[0040] (3) Before transportation, make a transportation record, specify the transportation conditions, date, arrival place and other conditions that need to be explained, and sign by the carrier (at the same time, ensure that the samples are sent to the laboratory as soon as possible under conditions close to the original storage temperature test);

[0041] (4) Prepare LB solid medium: the formula is tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L, aga...

Embodiment 2

[0049] The method for rapid high-throughput screening of oil recovery microorganisms in this embodiment comprises the following steps:

[0050] (1) Open the sampling port to release the near-end oil sample or water sample, (after a period of time) bring the pre-sterilized sampling container close to the sampling port, and take a sufficient amount of oil sample or water sample;

[0051] (2) After all samples are collected, they are directly sealed and labeled, and sealed with plastic bags (to ensure minimum external pollution);

[0052] (3) Before transportation, make a transportation record, specify the transportation conditions, date, arrival place and other conditions that need to be explained, and sign by the carrier (at the same time, ensure that the samples are sent to the laboratory as soon as possible under conditions close to the original storage temperature test);

[0053] (4) Preparation of LB solid medium: the formula is tryptone 12g / L, yeast extract 6g / L, NaCl 12g...

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Abstract

The invention discloses a rapid high-throughput screening method for oil-recovery microorganisms, belonging to the technical field of microbial enhanced oil recovery. According to the scheme I, the method comprises the following steps: (1) diluting a collected oil sample or water sample, coating an LB solid culture medium by virtue of the diluted oil sample or water sample, and culturing at 35-40 DEG C for 10-14 hours; and (2) inoculating a selected single microflora to an inorganic salt culture medium, adding liquid paraffin containing dyes, culturing at 35-40 DEG C for 4-48 hours, and observing whether a paraffin degradation phenomenon or oil extraction ring exists, if yes, the microorganisms are paraffin degrading bacteria or biosurfactant producing bacteria. According to the scheme II, the method comprises the following steps: (1) being same as the step (1) of the scheme I; (2) inoculating the selected single microflora to an LB liquid culture medium, carrying out fermentation cultivation, removing thalli to obtain clear liquid, and adding the dyes into the clear liquid; and (3) adding the clear liquid containing the dyes into intermediates of plant oil, standing, and observing whether the plant oil diffuses, if yes, the microorganisms are biosurfactant producing bacteria.

Description

technical field [0001] The invention relates to a method for rapid and high-throughput screening of oil recovery microorganisms, belonging to the technical field of microbial oil recovery. Background technique [0002] Microbial oil recovery is an enhanced oil recovery technology that has been widely studied and has high technical content in the field of petroleum engineering at home and abroad. The migration of microbial cells, microbial nutrient solution, and microbial metabolites in the oil layer, and the changes in the physical properties of rocks, oil, gas, and water caused by the interaction with rocks, oil, gas, and water in the reservoir. Microbial oil recovery is a cheap and effective oil recovery technology with simple construction and low cost. It is expected to become one of the main technologies for stabilizing oil, controlling water and improving oil recovery in the later stages of oilfield development. In recent years, with the support of the national high-te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 周志成张宇赵静付娜李晓晨王娟娟韩雨曦
Owner CHINA PETROLEUM & CHEM CORP
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