Method for construction of discrimination model for discriminating daqu quality

A discriminant model and technology of Daqu, which is applied in the field of Daqu quality identification, can solve the problems of cumbersome experimental operation, high cost, and difficulty in effectively improving the discrimination rate, and achieve the effect of high discrimination accuracy and simple operation and use

Active Publication Date: 2015-02-25
KWEICHOW MOUTAI COMPANY
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Problems solved by technology

[0005] For example, the Chinese invention patent with the patent number of 201310201060.5 and the patent name of "a method for constructing a discriminant model to identify the quality of Daqu" provides a method for identifying the quality of Daqu by using solid-phase microextraction and discriminant analysis. Daqu quality evaluation, this method is costly and the accuracy rate is unacceptable relative to its cost
In the Chinese invention patent with the patent number 200910228701.X and the patent name "A Quality Control Method for the Production of Maotai-flavored Liquor Daqu", a DGGE method using Daq

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  • Method for construction of discrimination model for discriminating daqu quality
  • Method for construction of discrimination model for discriminating daqu quality
  • Method for construction of discrimination model for discriminating daqu quality

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experiment example 1

[0044] 1. Sample information

[0045] The samples in this example come from 113 high-temperature Daqu provided by Kweichow Moutai Co., Ltd., and the samples include:

[0046] Modeling samples: 37 pieces of finished song, 21 pieces of out-of-warehouse yellow koji, and 20 out-of-warehouse white koji;

[0047] Test samples: 20 finished koji, 8 out-of-warehouse yellow koji, and 7 out-of-warehouse white koji.

[0048] 2. Information Extraction

[0049] Using 454 high-throughput sequencing technology, all samples were sequenced and analyzed, and a total of 580 types of microbial species (microbial numbers x1, x2, x3,..., x580) and their relative content were obtained.

[0050] 3. Variable screening and optimization

[0051] Input the variable and sample information into SIMCA-P software, use the partial least square method to screen and optimize the obtained microbial variables, according to the principle of variable projection importance index (VIP) value ≥ 1, such as figure 1 ...

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Abstract

The invention provides a method for construction of a discrimination model for discriminating daqu quality, the method comprises the following steps: 1, information extraction; 2, variable screening and optimization; 3, construction of the discrimination model; 4, verification of the model; and 5, discriminating; and the method is applied to discriminating of daqu quality in distiller's yeast. The method uses 454 high throughput sequencing technology, and solves the problem that the first generation sequencing technology cannot comprehensively and accurately reflect the composition information of daqu microbes; by use of partial least squares method for variable screening and optimization of the microbes and meanwhile combination with quadratic discriminatory analysis, the daqu quality discriminating evaluation is constructed, and is applied to discriminating of daqu quality grade, the method for discriminating finished product qu and out-taken yellow qu and white qu grade is constructed, and the method has the advantages of simple operation and use, higher discriminating accuracy rate, and the like.

Description

technical field [0001] The invention relates to the technical field of quality identification of Daqu, in particular to a method for realizing the quality identification of Daqu by applying the microbial composition information of Daqu and combining multivariate statistical methods. Background technique [0002] Daqu is an indispensable part of liquor brewing, which plays the roles of ①inoculating microorganisms; ②producing enzyme systems; ③providing flavor substances and precursors and ④supplementing materials. The first three functions are closely related to the floristic structure of the microbial community in Daqu, so the difference in microbial composition in Daqu can reflect its quality to a certain extent. [0003] The 454 high-throughput sequencing method is a new generation of DNA sequencing technology developed in recent years. With its digital signal, high data throughput, high sequencing depth, and high accuracy, it can realize large-scale multi-sample community ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
CPCC12Q1/6869G16B5/00C12Q2535/122
Inventor 王莉杨帆韩莹刘华陈良强王和玉汪地强金桃徐进
Owner KWEICHOW MOUTAI COMPANY
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