A Method of Identifying the Quality of Daqu Based on Box Plot

Abstract

The invention relates to the technical field of quality identification of a yeast for making hard liquor and particularly relates to a method for identifying the quality of the yeast for making hard liquor on the basis of a box plot. The method disclosed by the invention establishes the quality grade identification of the yeast for making hard liquor by screening variables through a microbe occurrence frequency and relative content method in combination with the box plot and can carry out grade identification on unknown sorted samples; a model has the characteristics of simple method and idea, easy and convenient operation, high feasibility and high identification accuracy rate.

Description

technical field [0001] The invention relates to the technical field of Daqu quality identification, in particular to a method for identifying the quality of Daqu based on a box plot. Background technique [0002] Daqu is an essential raw material for the liquor brewing process. It is known as the "bone of wine" and plays an extremely important role. Its functions include providing microbial sources, microbial enzyme sources, flavor substances and their prerequisite substances and supplementary materials in the brewing process. The first three functions are closely related to the microbial community structure in Daqu. Therefore, the microbial composition of Daqu The difference can reflect the quality of Daqu to a certain extent. [0003] The 454 high-throughput sequencing method is a new generation of DNA sequencing technology developed in recent years. It is famous for its digital signal, high data throughput, high sequencing depth, and high accuracy. It can realize large-s...

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Problems solved by technology

[0005] For the method of identifying the quality of Daqu, some researchers have done a lot of research, such as "A Method for Identifying the Quality of Daqu" with a patent number of 201310201060.5. The method described is to use solid-phase microextraction and discriminant analysis to identify the quality of Daqu, which is The quality of Daqu is evaluated by the flavor substances of Daqu; another example is the "Quality Control Method for the Production of Maotai-flavored Liquor Daqu" whose patent number is 200910228701. Qualified Daqu products are obtained by comparing the measured spectrum with the standard spectrum. In this scheme, DGGE technology can only detect more than a dozen dominant bacteria in the sample; if you want to know the specific bacterial species information, you need to clone and sequence, and the experimental operation is cumbersome. ; and this method cannot reflect the abundance of microorganisms. In addition, the map comparison in this scheme is only a simple comparison, and no relevant model has been established for comparison analysis

[0006] It can be seen that traditional Daqu quality inspection and control cannot use unknown category sample information to establish standards to determine the quality level of unknown Daqu samples. At the same time, the method of using 454 high-throughput sequencing technology combined with box plots to identify the quality of Daqu Not yet reported in the prior art

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