A Method of Identifying the Quality of Daqu Based on Box Plot

A technique of box plot and Daqu, which is applied in the field of quality identification of Daqu, can solve the problems of cumbersome experimental operation, inability to reflect the abundance of microorganisms, and lack of establishment of relevant models for comparison and analysis, and achieve high discrimination accuracy, simple operation, and method The effect of simple thinking

Active Publication Date: 2016-02-03
KWEICHOW MOUTAI COMPANY
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  • Claims
  • Application Information

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Problems solved by technology

[0005] For the method of identifying the quality of Daqu, some researchers have done a lot of research, such as "A Method for Identifying the Quality of Daqu" with a patent number of 201310201060.5. The method described is to use solid-phase microextraction and discriminant analysis to identify the quality of Daqu, which is The quality of Daqu is evaluated by the flavor substances of Daqu; another example is the "Quality Control Method for the Production of Maotai-flavored Liquor Daqu" whose patent number is 200910228701. Qualified Daqu products are obtained by comparing the measured spectrum with the standard spectrum. In this scheme, DGGE technology can only detect more than a dozen dominant bacteria in the sample; if you want to know the specific bacterial species information, you need to clone and sequence, and the experimental operation is cumbersome. ; and this method cannot reflect the abundance of microorganisms. In addition, the map comparison in this scheme is only a simple comparison, and no relevant model has been established for comparison analysis
[0006] It can be seen that traditional Daqu quality inspection and control cannot use unknown category sample information to establish standards to determine the quality level of unknown Daqu samples. At the same time, the method of using 454 high-throughput sequencing technology combined with box plots to identify the quality of Daqu Not yet reported in the prior art

Method used

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  • A Method of Identifying the Quality of Daqu Based on Box Plot
  • A Method of Identifying the Quality of Daqu Based on Box Plot
  • A Method of Identifying the Quality of Daqu Based on Box Plot

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Embodiment

[0031] Such as figure 1 and figure 2 As shown, a method for identifying the quality of Daqu based on the box plot includes the following steps:

[0032] (1) Daqu sample acquisition and grouping

[0033] The samples are all high-temperature Daqu from Kweichow Moutai Co., Ltd. There are 149 samples in total, of which 120 samples are used for modeling, and the remaining 29 samples are used for model testing and verification.

[0034] (2) Daqu sample information acquisition

[0035] Using 454 high-throughput sequencing technology, the samples were sequenced and analyzed, and a total of 580 types of microorganisms were obtained (microbial numbers are x1, x2, x3, x4, x5, x6, x7, x8, x9, x10, x11, x12, x13, x14, x15, x16, x17, x18, x19, x20, x21, x22, x23, x24, x25, x26, x27, x28, x568, x569, x570, x571, x572, x573, x574, x575, x576, x577, x578, x579, x580) and the relative content of each microorganism, and make records.

[0036] (3) Screening of microbial variables

[0037] ...

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Abstract

The invention relates to the technical field of quality identification of a yeast for making hard liquor and particularly relates to a method for identifying the quality of the yeast for making hard liquor on the basis of a box plot. The method disclosed by the invention establishes the quality grade identification of the yeast for making hard liquor by screening variables through a microbe occurrence frequency and relative content method in combination with the box plot and can carry out grade identification on unknown sorted samples; a model has the characteristics of simple method and idea, easy and convenient operation, high feasibility and high identification accuracy rate.

Description

technical field [0001] The invention relates to the technical field of Daqu quality identification, in particular to a method for identifying the quality of Daqu based on a box plot. Background technique [0002] Daqu is an essential raw material for the liquor brewing process. It is known as the "bone of wine" and plays an extremely important role. Its functions include providing microbial sources, microbial enzyme sources, flavor substances and their prerequisite substances and supplementary materials in the brewing process. The first three functions are closely related to the microbial community structure in Daqu. Therefore, the microbial composition of Daqu The difference can reflect the quality of Daqu to a certain extent. [0003] The 454 high-throughput sequencing method is a new generation of DNA sequencing technology developed in recent years. It is famous for its digital signal, high data throughput, high sequencing depth, and high accuracy. It can realize large-s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/06
CPCC12Q1/6869C12Q2535/122C12Q2537/165
Inventor 杨帆王莉陈良强罗汝叶胡芳精李靖圆王和玉汪地强王亚玉
Owner KWEICHOW MOUTAI COMPANY
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