Preparation method of anti-myocardial antibody quadruple diagnostic kit

A diagnostic kit, myocardial antibody technology, applied in biological testing, material testing products, measuring devices, etc., can solve problems such as difficult long-term storage, limited clinical application of myocardial antibody detection, and difficulty in separation and purification of natural myocardial antigens, so as to improve diagnosis levels, reducing readmission rates and mortality, and achieving early diagnosis

Active Publication Date: 2015-03-11
WUHAN HUAJIYUAN BIOTECH DEV
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AI Technical Summary

Problems solved by technology

[0005] The separation and purification of natural myocardial antigens is difficult, and it is difficult to preserve for a long time, which limits the clinical application of myocardial antibody detection. According to the amino acid sequence of myocardial autoantibodies and the distribution of antigenic determinants, the peptides of natural antigenic determinants are designed and synthesized, and the peptides are used as Antigen detection of anti-cardiopeptide antibodies in patients

Method used

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  • Preparation method of anti-myocardial antibody quadruple diagnostic kit
  • Preparation method of anti-myocardial antibody quadruple diagnostic kit
  • Preparation method of anti-myocardial antibody quadruple diagnostic kit

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Embodiment Construction

[0023] Below in conjunction with embodiment further illustrate preparation method of the present invention

[0024] Preparation of kit detection reagents

[0025] 1. Synthesis of Antigenic Peptides

[0026] 1. Synthesize antigenic peptides with the same antigenicity as four myocardial antigens: anti-myocardial mitochondrial adenylyltransferase (ANT), anti-β1 adrenergic receptor, anti-calcium ion channel and anti-myocardial myosin heavy chain ( MHC). The amino acid sequence of the corresponding cardiomyocyte antigen peptide is as follows:

[0027] Amino acid sequence of synthetic anti-myocardial mitochondrial adenylyltransferase (ANT) peptide:

[0028] ANT-1

[0029] Pro-Ile-Glu-Arg-Val-Lys-Leu-Leu-Leu-Gln-Cys-Cys

[0030] ANT-2 Tyr-Asp-Glu-Ile-Lys-Lys-Phe-Val-Cys-Cys

[0031] synthetic anti-beta 1 Amino acid sequence of adrenergic receptor peptide:

[0032] His-Trp-Trp-Arg-Ala-Glu-Ser-Asp-Glu-Ala-Arg-Arg-Cys-Tyr-Asn-Asp-Pro-Lys-Cys-Cys-Asp-Phe-Val-The-Asn- Cys-Cys

...

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Abstract

The invention discloses a preparation method of an anti-myocardial antibody quadruple diagnostic kit. The anti-myocardial antibody quadruple diagnostic kit provided by the invention is formed by respectively coating four segments of anti-myocardial antibody peptides, namely an anti-myocardial mitochondrial adenosine transferase antibody, a beta1 adrenoceptor antibody, an anti-calcium channel antibody and a cardiac myosin heavy chain antibody. The kit provided by the invention is used to detect an anti-cardiomyopeptidin antibody in serum of a suspected DCM patient. By the kit, diagnosis level of DCM can be improved, and early diagnosis is realized.

Description

technical field [0001] The invention belongs to the field of medical detection reagents. It relates to the preparation of an anti-myocardial antibody quadruple diagnostic kit. Anti-myocardial antibody quadruple diagnostic kit can be used to assist in the diagnosis and treatment of viral myocarditis and dilated cardiomyopathy. Background technique [0002] Viral heart disease (VHD) is a group of heart diseases related to viral infection, the common ones are viral myocarditis (VMC) and dilated cardiomyopathy (DCM). In recent years, the incidence of VHD has gradually increased. During the epidemic period of viral infection, myocarditis occurs in about 5% of patients, and 12.5% ​​of them can be transformed into DCM, while the incidence of DCM in the general population is only 8-10 / 100,000 population. The census prevalence rate of DCM among 66,632 people in Nanning, Guangxi, my country was 84.04 / 100,000, accounting for the fourth place in the natural incidence of various heart...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893
Inventor 廖玉华王敏汪朝晖袁璟程翔
Owner WUHAN HUAJIYUAN BIOTECH DEV
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