Preparative circulating chromatography apparatus

Abstract

A preparative circulating chromatography apparatus can be applied in chromatographic separation preparation of target components in a complex sample and is mainly advantaged in that: (1) with pre-treatment and concentration of an on-line sample, large-volume sampling on the premise of a high column efficiency can be achieved; (2) through position exchange between a separation column and a trapping column, circulating separation and purification of the target components are achieved and the preparative circulating chromatography apparatus is less in circulating dead volume and column efficiency loss; and (3) through concentration-elution of a finish product through the trapping column, a separation-concentration integrated process is achieved, wherein the three functions are achieved through adjustment of an adsorption performance of the trapping column and valve exchange. Compared with a conventional preparative chromatography method, the apparatus has significant advantages on separations of natural products, drugs and chemical products, especially on separation and preparation of micro-scale targeted components and unstable components in a mixture.

Description

technical field [0001] The invention is a circulating preparative liquid chromatography device for complex sample separation. Background technique [0002] In the separation and preparation of natural products, drugs or chemical products, preparative liquid chromatography is the most common and effective separation method. However, there are still many problems in the application of this technology: (1) The composition of the target substance is The content in the mixed sample is very low, and there are too many components other than the target component; (2) The target component is unstable due to cumbersome transfer process or environmental factors such as light, heat, and oxygen during the pretreatment or drying process of the sample (3) The sample solubility is poor, resulting in a small sample volume, a waste of solvent, or volume overload when a large volume sample is loaded, which affects the separation effect; (4) The sample is dissolved in a solvent with strong elut...

AI Technical Summary

Problems solved by technology

[0003] Existing technologies are often only applicable to the application of analytical chromatography, but are not practical for preparative equipment, and the transformation of technology often requires a large amount of investment

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