A kind of preparation method of fresh matsutake active substance
A technology for active substances and matsutake mushrooms, which is applied in the field of preparation of matsutake mushroom active substances, can solve the problems of inability to reduce resource waste, inability to utilize matsutake and other external products and scraps, etc., and achieves a technology that improves utilization value and economic benefits, is simple to use, and is easy to preserve. Effect
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specific Embodiment approach 1
[0024] Specific embodiment one: the preparation method of a kind of fresh matsutake active substance of the present embodiment, it is carried out according to the following steps:
[0025] 1. Select fresh, disease-free and insect-free matsutake, wash it in clear water, and drain; slice the matsutake fruiting body; add 20 times its volume of sterile distilled water, and soak the sliced matsutake under reduced pressure in the containing 0.03% by mass of citric acid, 0.02% by mass of n-butanol and 0.02% by mass of myristyl ester in a mixed solution, after vacuum immersion for 10 minutes, add 0.02% by mass of %EDTA and mass percentage content are 0.2% sodium citrate, then smash matsutake tissue, then add 6-7g / L cellulase, 6-7g pectinase / L and 6-7g / L papain to carry out enzymolysis reaction to obtain matsutake processed raw materials; wherein, the enzymolysis reaction conditions are pH=5, temperature is 50°C, enzymolysis time is 1.5h, enzyme and substrate The substance concentra...
specific Embodiment approach 2
[0033] Specific embodiment two: the difference between this embodiment and specific embodiment one is: in step one, add the cellulase of 6.8g / L, the pectinase of 6.8g / L in the ratio of 1:1:1.4 by volume Carry out enzymolysis reaction with the papain of 6.8g / L. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0034] Embodiment 3: This embodiment differs from Embodiment 1 in that the ultrasonic treatment conditions in step 2 are: sonic power 100-150w, extraction time 5-10min, ultrasonic working time 2-4s, and interval time 1-3s. Others are the same as in the first embodiment.
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