Preparation method of high-purity refined salinomycin sodium salt

Abstract

The invention relates to a preparation method of high-purity refined salinomycin sodium salt. The high-purity refined salinomycin sodium salt is prepared from salinomycin fermentation liquor prepared by the prior art, and the preparation method comprises the following steps: regulating pH value by virtue of sodium hydroxide so as to generate sodium salt of salinomycin, removing water in the fermentation liquor by virtue of plate-frame pressure filtration, then extracting salinomycin sodium from mycelia by virtue of methanol, and carrying out a series of processing steps including concentrating, crystallizing, refining and drying, so that the refined salinomycin sodium is prepared. The preparation method disclosed by the invention can be used for extracting the active ingredients of the salinomycin from the fermentation liquor obtained in the production process, effectively removing remaining medium residues, water and other possible impurities in the production process, improving the yield and purity of the product, relieving possible toxic reaction generated due to the existence of other impurities in a process of using the product and enhancing the safety of medication.

Description

technical field [0001] The invention relates to the production field of biofermentation pharmaceutical products, in particular to a preparation method of high-purity salinomycin sodium refined product. Background technique [0002] Salinomycin sodium is a monovalent polyether ionophore anticoccidial drug with broad-spectrum anticoccidial effect. The mechanism of its coccidial action is to allow a large amount of Na+ to enter the cell by interfering with the normal penetration of K+ and Na+ in the coccidian cells. In order to balance the osmotic pressure, a large amount of water enters the coccidia cells, causing swelling and death. It has effects on chicken poisonous, tender, giant, moderate, heap type, brucei and other Eimeria, especially the strongest effect on giant and brucei. It has obvious effect on sporozoites, first and second generation merozoites of chicken coccidia. In addition, salinomycin can promote the growth of animals. Feeding 30-60mg / kg drug material to ...

AI Technical Summary

Problems solved by technology

[0005] Salinomycin is synthesized through the secondary metabolism of production bacteria by using soybean oil, white sugar, soybean cake powder, ammonium sulfate, potassium dihydrogen phosphate, potassium chloride and other food and chemical raw materials through biological fermentation. , because salinomycin is insoluble in water, it exists in the mycelium of the producing bacteria, mixed with mycelium, residual medium, etc., and it is impossible to directly obtain salinomycin products

[0006] The main application form of salinomycin sodium products is premix. At present, the industry mainly adopts the method of directly processing the fermentation liquid after fermentation to obtain the premix containing salinomycin, instead of extracting the refined salinomycin and then mixing it for production. Therefore, there is no production of salinomycin sodium in the process. Although this method has advantages in environmental protection and other aspects, there are hidden dangers in aspects such as safe drug use and product purity control, and it cannot meet other requirements for use when pure products are required for this product.