Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Diagnostic kit for distinguishing active and latent mycobacterium tuberculosis infection

A technology of mycobacterium tuberculosis and diagnostic kits, applied in the field of biomedicine, can solve problems such as unrelated research, and achieve the effects of rapid operation, improved diagnostic efficiency, high sensitivity and accuracy

Inactive Publication Date: 2015-03-25
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
View PDF5 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CTLA-4 molecule is involved in the immune response of active tuberculosis. There are related studies on the expression of CTLA-4 molecule in T cells and the difference of its gene polymorphism between active Mycobacterium tuberculosis infection and healthy people, but in There are no relevant studies at home and abroad on active and latent Mycobacterium tuberculosis infection population

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Diagnostic kit for distinguishing active and latent mycobacterium tuberculosis infection
  • Diagnostic kit for distinguishing active and latent mycobacterium tuberculosis infection
  • Diagnostic kit for distinguishing active and latent mycobacterium tuberculosis infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034]The invention provides a diagnostic kit for distinguishing active and latent Mycobacterium tuberculosis infection by using CTLA-4 and a method for using the diagnostic kit to distinguish active and latent Mycobacterium tuberculosis infection.

[0035] In the following, the present invention will be described in detail and specifically through specific examples, so as to better understand the present invention, but the following examples do not limit the scope of the present invention.

[0036] In this embodiment, a diagnostic kit for distinguishing active and latent Mycobacterium tuberculosis infection is provided, the diagnostic kit includes CTLA-4, CD3, CD4, CD25, FoxP3 fluorescent antibodies; red blood cell lysate, membrane rupture fluid, permwash , wash buffer and flow tubes, etc.

[0037] In this embodiment, the method for identifying active and latent Mycobacterium tuberculosis infection using the above-mentioned diagnostic kit to identify markers includes the foll...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a diagnostic kit for distinguishing active and latent mycobacterium tuberculosis infection with cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) serving as a diagnosis marker and a method for distinguishing active and latent mycobacterium tuberculosis infection by means of the diagnostic kit. The diagnostic kit comprises a CTLA-4, CD3, CD4, CD25, FoxP3 fluorescent antibody, erythrocyte lysate, membrane breaking liquid, washing buffer, phosphate buffer, fetal calf serum, stationary liquid and a streaming pipe. The diagnostic kit can be used for detecting the CTLA-4 expression quantity of peripheral venous blood of a patient and distinguishing active and latent mycobacterium tuberculosis infection, sensitivity and accuracy are high, and a strong basis is provided for clinic differential diagnosis.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to a method for distinguishing active and latent tuberculosis using CD4+CD25+FoxP3+T cell cytotoxic T lymphocyte-associated antigen 4 (CTLA-4). A diagnostic kit for mycobacterial infection and a method for distinguishing active from latent Mycobacterium tuberculosis infection using the diagnostic kit. Background technique [0002] Tuberculosis is an infectious disease that seriously endangers human health and is currently the chronic infectious disease with the highest mortality rate in the world. In the 1980s, people once believed that the disease had been controlled, but in recent years, the incidence and mortality of tuberculosis have been on the rise. According to the latest report on tuberculosis from the World Health Organization, the current global average number of patients is 14 million. There are 8.8 million new active tuberculosis patients, an average of 1.1 million HI...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/569
CPCG01N33/5695
Inventor 邵凌云高岩张文宏张冰琰吴晶陈嘉臻王森
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com