Diagnostic kit for distinguishing active and latent mycobacterium tuberculosis infection

A technology of mycobacterium tuberculosis and diagnostic kits, applied in the field of biomedicine, can solve problems such as unrelated research, and achieve the effects of rapid operation, improved diagnostic efficiency, high sensitivity and accuracy

Inactive Publication Date: 2015-03-25
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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Problems solved by technology

CTLA-4 molecule is involved in the immune response of active tuberculosis. There are related studies on the expression of CTLA-4 molecule in T cells and the difference of its gene polymorphism be

Method used

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  • Diagnostic kit for distinguishing active and latent mycobacterium tuberculosis infection
  • Diagnostic kit for distinguishing active and latent mycobacterium tuberculosis infection
  • Diagnostic kit for distinguishing active and latent mycobacterium tuberculosis infection

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Embodiment Construction

[0034]The invention provides a diagnostic kit for distinguishing active and latent Mycobacterium tuberculosis infection by using CTLA-4 and a method for using the diagnostic kit to distinguish active and latent Mycobacterium tuberculosis infection.

[0035] In the following, the present invention will be described in detail and specifically through specific examples, so as to better understand the present invention, but the following examples do not limit the scope of the present invention.

[0036] In this embodiment, a diagnostic kit for distinguishing active and latent Mycobacterium tuberculosis infection is provided, the diagnostic kit includes CTLA-4, CD3, CD4, CD25, FoxP3 fluorescent antibodies; red blood cell lysate, membrane rupture fluid, permwash , wash buffer and flow tubes, etc.

[0037] In this embodiment, the method for identifying active and latent Mycobacterium tuberculosis infection using the above-mentioned diagnostic kit to identify markers includes the foll...

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Abstract

The invention provides a diagnostic kit for distinguishing active and latent mycobacterium tuberculosis infection with cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) serving as a diagnosis marker and a method for distinguishing active and latent mycobacterium tuberculosis infection by means of the diagnostic kit. The diagnostic kit comprises a CTLA-4, CD3, CD4, CD25, FoxP3 fluorescent antibody, erythrocyte lysate, membrane breaking liquid, washing buffer, phosphate buffer, fetal calf serum, stationary liquid and a streaming pipe. The diagnostic kit can be used for detecting the CTLA-4 expression quantity of peripheral venous blood of a patient and distinguishing active and latent mycobacterium tuberculosis infection, sensitivity and accuracy are high, and a strong basis is provided for clinic differential diagnosis.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to a method for distinguishing active and latent tuberculosis using CD4+CD25+FoxP3+T cell cytotoxic T lymphocyte-associated antigen 4 (CTLA-4). A diagnostic kit for mycobacterial infection and a method for distinguishing active from latent Mycobacterium tuberculosis infection using the diagnostic kit. Background technique [0002] Tuberculosis is an infectious disease that seriously endangers human health and is currently the chronic infectious disease with the highest mortality rate in the world. In the 1980s, people once believed that the disease had been controlled, but in recent years, the incidence and mortality of tuberculosis have been on the rise. According to the latest report on tuberculosis from the World Health Organization, the current global average number of patients is 14 million. There are 8.8 million new active tuberculosis patients, an average of 1.1 million HI...

Claims

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Application Information

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IPC IPC(8): G01N33/569
CPCG01N33/5695
Inventor 邵凌云高岩张文宏张冰琰吴晶陈嘉臻王森
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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