A kind of animal bifidobacteria and composition thereof

A technology of animal bifidobacterium and composition, applied in the direction of drug combination, bacteria, microorganisms, etc., can solve the problems of unsolved room temperature storage, easy inactivation, etc., achieve good synergistic growth, high number of viable bacteria, and regulate intestinal tract The effect of microbiota

Active Publication Date: 2017-04-05
HANGZHOU GRAND BIOLOGIC PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Strains are easily inactivated during production, transportation and storage
In order to keep the number of viable bacteria in the product at a certain level, researchers at home and abroad have done a lot of research on genetic modification, microencapsulation technology, and bacteria embedding technology. Stored at low temperature, most products containing bifidobacteria have not solved the problem of room temperature storage

Method used

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  • A kind of animal bifidobacteria and composition thereof
  • A kind of animal bifidobacteria and composition thereof
  • A kind of animal bifidobacteria and composition thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The acquisition of embodiment 1 bacterial strain

[0027] Bifidobacterium animalis, isolated from healthy human intestines in Jianggan District, Hangzhou City, and screened out strains suitable for human body, Bifidobacterium animalis has been preserved in the General Microbiology Center of Institute of Microbiology, Chinese Academy of Sciences on December 29, 2010, with the preservation number It is CGMCC No.4521.

[0028] Bifidobacterium animalis was collected from 20 healthy children aged 2-6 years old in Jianggan District, Hangzhou City, 10 males and 10 males, all of whom had no history of gastrointestinal diseases and did not take any antibacterial drugs within two weeks before sampling. When sampling, use a sterile glass rod to pick up 4-10g of naturally discharged fresh feces, place them in a sterile plate, quickly put them into an anaerobic tank, and bring them back to the laboratory for separation.

Embodiment 2

[0029] Identification and cultivation of embodiment 2 bacterial strains

[0030] 2.1 Use the dilution coating method to separate and purify the collected strain samples. After purification and culture, observe the colony morphology, take fresh culture, inoculate sugar fermentation test tube, and identify the biochemical reaction characteristics of the strain. At the same time, take the fresh culture of the strain to be tested, use the API 20A biochemical identification strip of the French Mérieux company, and refer to the biochemical identification database of the Mérieux company for API biochemical identification of the strain to be tested. At the same time, the 16S rRNA of the isolated and purified strain will be extracted and sequenced, and the sequencing results will be compared with the existing sequences in NCBI's GeneBank. The isolated and purified strains were stored at 2-8°C.

[0031] After identification, the colonies of the strains to be tested are characterized b...

Embodiment 3

[0047] Inoculate the qualified animal bifidobacterium seed liquid into the fermentation medium in the fermenter, and perform anaerobic culture at 36-38° C. for 16-20 hours. During the cultivation process, samples were taken for microscopic inspection, and the pH value was measured. After the cultivation, samples were taken for pure bacteria inspection ( figure 1 ). Gastric acid and bile salt resistance test of the strain of embodiment 3

[0048] 3.1 Bile salt tolerance test

[0049] The composition of the improved TPY nutrient solution used for cultivating bacterial strains is:

[0050] The isolated and purified bacterial strains were cultured strictly anaerobically at 37° C. for 15 h with sterilized modified TPY medium. The activated strain culture solution was inserted into the aseptic modified TPY liquid medium containing different bile salt concentrations with 10% inoculum (the bile salt concentration was 1%, 2%, 3%, 4%, 5%, 6%, 7% ), while the improved TPY medium with...

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Abstract

The invention discloses an animal bifidobacterium and a composition thereof, belonging to the technical field of microorganism application. The preservation number of animal Bifidobacteria provided by the present invention is CGMCC No.4521, which was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms in Beijing on December 29, 2010. Animal Bifidobacterium provided by the present invention has synergistic growth effect with Lactobacillus casei, Streptococcus faecalis and Bacillus cereus, and has tolerance to quinolones, aminoglycosides and monocyclic β-lactam antibiotics, Suitable for food, medicine and / or health products.

Description

technical field [0001] The invention relates to an animal bifidobacterium and a composition thereof, belonging to the technical field of microorganism application. [0002] technical background [0003] Bifidobacterium animalis is the earliest physiological bacteria discovered by human beings, and it is a typical beneficial bacteria in the human intestinal tract. Bifidobacterium animalis is a probiotic colonized in the human intestinal tract. Its existence can effectively reduce the pH of the human body system, change the intestinal flora, optimize the intestinal microecology, inhibit and kill pathogenic bacteria, thereby enhancing human immunity. Improve the body's disease resistance, and at the same time, animal bifidobacteria can also synthesize multivitamins in the intestinal tract. In addition, Bifidobacterium animalis also has laxative effect, lowering cholesterol and triglyceride, inhibiting cancer cells, anti-aging and so on. Therefore, the quantity and quality of b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/745A61P1/14C12R1/01
CPCA61K35/745C12N1/205C12R2001/01
Inventor 曹勇闫天文
Owner HANGZHOU GRAND BIOLOGIC PHARMA INC
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