Acquisition and application of strain for treating EH oil-containing wastewater microorganisms

A microbial strain and microbial technology, which is applied in the field of acquisition and application of microbial strains for treating EH oil-containing wastewater, can solve the problems of failing to meet the standards and the small amount of microorganisms that can treat EH oil, and achieve an environmentally friendly effect

Inactive Publication Date: 2015-04-08
STATE GRID CORP OF CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Most of the existing sewage treatment in power plants adopts the A / O process. Generally speaking, this process can make the waste water discharge up to standard after treatment, but the waste water containing EH oil cannot meet the standard after treatment. This is due to the existence of treatable EH in the A / O system Oil has little microbial inventory

Method used

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Embodiment Construction

[0020] The implementation of the present invention will be described in further detail below. The following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0021] A kind of acquisition that handles EH oil-containing wastewater microbial strains, the method comprises the steps as follows:

[0022] ① Determine the existence and separation of microorganisms in the treated EH oil;

[0023] ② Whichever expresses the DNA molecule;

[0024] ③ Obtain microbial parent strains that are easy to survive in large quantities in the wastewater treatment system as carriers;

[0025] ④ Implant the expression DNA into the parent strain to obtain a new microbial DNW;

[0026] ⑤Acclimate DNW and carry out certain bio-augmentation training to become applicable microbial bacteria DNW N ;

[0027] A method for using the above-mentioned microbial strains for treating waste water containing EH oil, the specific steps include:

[00...

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Abstract

The invention relates to acquisition and application of a strain for treating EH oil-containing wastewater microorganisms. The acquisition comprises the following steps: (1) determining existence of EH oil microorganisms, and separating; (2) extracting an expression DNA molecule of the EH oil microorganisms; (3) taking massive living microorganism parent strains in a wastewater treatment system as a carrier; (4) planting the expression DNA into the parent strains to obtain new microorganism DNW; and (5) domesticating the DNW and carrying out a certain biological enrichment culture to obtain applicable microorganism bacteria. The application comprises (1) addition of DNWN in the anaerobic phase when A / O is used for treating EH oil-containing wastewater; (2) addition of 300g of DNWN of microorganism bacteria when the dosage is 10m<3> / h for output; and (3) stable work of DNWN after 4 hours. By using the treatment method, the removal rate is not less than 85 percent when the EH oil content is less than 600mu g / L. The acquisition and application are environmentally friendly, and do not have variation.

Description

technical field [0001] The invention belongs to the technical field of environmental protection and waste water treatment, in particular to the acquisition and application of microbial strains for treating waste water containing EH oil. Background technique [0002] Most of the existing sewage treatment in power plants adopts the A / O process. Generally speaking, this process can make the waste water discharge up to standard after treatment, but the waste water containing EH oil cannot meet the standard after treatment. This is due to the existence of treatable EH in the A / O system Oil has a low microbial inventory. Contents of the invention [0003] The object of the present invention is to overcome the deficiencies of the prior art, and provide a kind of acquisition and application of microbial strains for treating EH oil-containing wastewater. [0004] The present invention solves its technical problem and realizes by taking the following technical solutions: [0005] ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/74C02F3/34
Inventor 邵林郝春艳赵鹏于金山
Owner STATE GRID CORP OF CHINA
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