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Gel filtration chromatographic analysis method for alkyl glucoside surfactant product components

A technology of gel filtration chromatography and surfactant, which is applied in the field of fine chemical product testing, can solve the problems of lack of reference substance preparation process, weak separation ability, and difficulty in obtaining the ratio information of single and polyglycosides, and achieve objective and reliable analysis results. Separation and classification of reasonable effects

Active Publication Date: 2015-04-08
CHINA RES INST OF DAILY CHEM IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The liquid chromatographic analysis methods of alkyl glycosides reported in the literature mostly use C 18 reversed-phase column for analysis, C 18 The separation mechanism of the column is mainly adsorption-distribution, which has a strong separation ability for hydrophobic substances, but insufficient retention for the highly hydrophilic polyglycoside components, and the separation ability is weak, and polyglycoside components with different alkyl groups often mix out. peaks, it is difficult to obtain information on the ratio of single to polyglycosides for different alkyl groups
In addition, the existing literature reports lack the preparation process of reference substances, so the identification of chromatographic peaks is subjective

Method used

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  • Gel filtration chromatographic analysis method for alkyl glucoside surfactant product components
  • Gel filtration chromatographic analysis method for alkyl glucoside surfactant product components
  • Gel filtration chromatographic analysis method for alkyl glucoside surfactant product components

Examples

Experimental program
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Effect test

Embodiment 1

[0032] C 8-10 Component Analysis of Alkyl Glycoside Products

[0033] (1) Preparation of monoglycoside and polyglycoside reference substance: take anhydrous C 8-10 Alkyl glucoside 2g, dissolved in 10ml of methanol-chloroform with a volume ratio of 1:1, the preparation concentration is 0.2g·mL -1 , loaded on a Sephadex LH-20 Sephadex column, eluted with the same solvent, the flow rate was controlled at about 5 seconds / drop, and the eluate was collected at 2ml per tube, classified and combined after identification, and concentrated to dryness to obtain C 8 Monoglycoside, C 10 Monoglycoside, C 8Polyglycosides and C 10 Polyglycoside four reference substances;

[0034] (2) Preparation of reference substance solution: Weigh 15.1 mg of monoglycoside reference substance and 12.3 mg of polyglycoside reference substance, add 10 ml of methanol-acetonitrile-water at a volume ratio of 1:1:8 to dissolve, and prepare a concentration of 1.51 mg·mL -1 The single glycoside reference subst...

Embodiment 2

[0046] C 12-14 Component Analysis of Alkyl Glycoside Products

[0047] (1) Preparation of monoglycoside and polyglycoside reference substance: take anhydrous C 12-14 Alkyl glucoside 1g, dissolved in 2ml of methanol-chloroform with a volume ratio of 1:1, the preparation concentration is 0.5g·mL -1 , load the sample on Sephadex LH-20 Sephadex column, elute with the same solvent, control the flow rate at about 7 seconds / drop, collect the eluate at 3ml per tube, classify and combine after identification, concentrate to dryness, and obtain C 12 Monoglycoside, C 14 Monoglycoside, C 12 Polyglycosides and C 14 Polyglycoside four reference substances;

[0048] (2) Preparation of reference substance solution: Weigh 20.4mg of monoglycoside reference substance and 15.2mg of polyglycoside reference substance respectively, add 10ml of methanol-acetonitrile-water at a volume ratio of 1:1:8 to dissolve, and prepare a concentration of 2.04mg·mL -1 The single glycoside reference substance...

Embodiment 3

[0054] C 16-18 Component Analysis of Alkyl Glycoside Products

[0055] (1) Preparation of monoglycoside and polyglycoside reference substance: take anhydrous C 16-18 Alkyl glucoside 5.0g, dissolved in 5ml of methanol-chloroform with a volume ratio of 1:1, the preparation concentration is 1.0g·mL -1 , load the sample on SephadexLH-20 Sephadex column, elute with the same solvent, control the flow rate at about 10 seconds / drop, collect the eluate at 4ml per tube, classify and combine after identification, concentrate to dryness, and obtain C 16 Monoglycoside, C 18 Monoglycoside, C 16 Polyglycosides and C 18 Polyglycoside four reference substances;

[0056] (2) Preparation of reference substance solution: Weigh 6.3 mg of monoglycoside reference substance and 6.4 mg of polyglycoside reference substance respectively, add 2 ml of methanol-acetonitrile-water with a volume ratio of 1:1:8 to dissolve, and prepare a concentration of 3.2 mg·mL -1 Monoglycoside, polyglycoside referen...

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Abstract

The invention relates to a gel filtration chromatographic analysis method for alkyl glucoside surfactant product components. The method includes: firstly preparing reference substances of monoside and polyglycoside, then adding a mobile phase into the reference substances and a to-be-detected sample to conduct dissolution respectively, then employing an evaporative light-scattering detector collocated high performance liquid chromatograph to conduct sample introduction respectively, carrying out separation on a gel filtration chromatographic column, taking methanol-acetonitrile-water as the mobile phase to carry out gradient elution, and conducting area normalization method by an area normalization technique. With the method provided by the invention, the analysis result can be more objective and reliable.

Description

technical field [0001] The invention belongs to the technical field of detection of fine chemical products, and in particular relates to an analysis method for components of alkyl glycoside surfactant products. Background technique [0002] Alkyl glycosides are a class of surfactants with long-chain alkyl groups as hydrophobic groups and sugar groups as hydrophilic groups, generally composed of C 8-10 、C 12-14 or C 16-18 The fatty alcohol and glucose are synthesized by condensation reaction, and the common product types can be divided into C according to different raw materials 8-10 Alkyl glycoside, C 12-14 Alkyl glycoside or C 16-18 Alkyl glycosides, etc. Alkyl glycosides use glucose and fatty alcohols derived from biomass resources as raw materials, and are typical green surfactants. With the concept of green life deeply rooted in the hearts of the people, the green and safety advantages of such products have been widely recognized, so in recent years alkyl The domes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 张军杨秀全白亮周媛
Owner CHINA RES INST OF DAILY CHEM IND
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