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A method for increasing the fucoxanthin content of Phaeodactylum tricornutum with ammonium cerium sulfate
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A technology of Phaeodactylum sp. fucoxanthin and ceric ammonium sulfate is applied in the field of biomedicine and achieves the effects of simple and convenient operation, increased content and low cost
Inactive Publication Date: 2017-11-14
NINGBO UNIV
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At present, there is no report on the use of various elicitors to increase the content of fucoxanthin
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Embodiment 1
[0036] Embodiment 1 The influence of ammonium cerium sulfate on fucoxanthin in Phaeodactylum tricornutum
[0037] Take 18 groups of 300ml sterilized MAD culture solution, respectively, and insert them into Phaeodactylum tricornutum algae at a ratio of 1:10. Use 0.2mg / L, 0.4mg / L, 0.8mg / L, 1.6mg / L, 2mg / L ammonium cerium sulfate to treat Phaeodactylum tricornutum and 3 parallel groups without any treatment. From the day when Phaeodactylum tricornutum was inserted into the culture medium, the concentration of each group of algae liquid was measured by spectrophotometer every day, and then measured for another 6 days.
[0038] The result is as figure 1 As shown, when different concentrations of cerium ammonium sulfate (CAS) were added, the absorbance value of each group did not change significantly. The OD value of algae treated with L ammonium cerium sulfate was the smallest. Wherein the OD value of the control group was 0.4322, and the OD value of the 0.4mg / L experimental grou...
Embodiment 2
[0040] Take 400ml of sterilized seawater for 7 groups, and add 400μl of MAD mother solution respectively. Add 40ml of Phaeodactylum tricornutum mother solution to the culture solution and cultivate in a light incubator. After 2 days of cultivation, different concentrations of cerium ammonium sulfate were added to the 6 groups of algae culture solutions, the concentrations were 0mg / L, 0.2mg / L, 0.4mg / L, 0.8mg / L, 1.6mg / L, 2.0mg / L, respectively. mg / L.
[0041] Such as figure 2 As shown, from the measurement of the growth curve of Phaeodactylum tricornutum, it can be known that Phaeodactylum tricornutum has reached the end of the logarithmic phase after the 6th day of cultivation, and the maximum biomass can be obtained. Therefore, it is necessary to collect the algae liquid when cultivating to the 6th day.
[0042]Take 80ml of each group of Phaeodactylum tricornutum into a centrifuge tube, centrifuge at 4°C, 5000rpm for 10min, discard the supernatant, take 1ml of MAD seawater ...
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Abstract
The invention discloses a method for increasing the fucoxanthin content of Phaeodactylum tricornutum with ammonium cerium sulfate. Sterilized seawater is mixed with MAD mother liquor to obtain a nutrient solution, and the Phaeodactylum tricornutum mother liquor is added to the nutrient solution for cultivation. After culturing for 2 days , add ammonium cerium sulfate, continue to cultivate for 6 days, collect the algae liquid, centrifuge, discard the supernatant, collect the algae body, freeze-dry the algae body for 2 days, and grind it for later use. Stand in the middle for 1h, 4°C, 13000rpm, and centrifuge for 10min to obtain the supernatant. The invention has the advantages of simple and convenient operation, low cost and short production period, and is suitable for batch processing and factory production.
Description
technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for increasing the content of fucoxanthin in Phaeodactylum tricornutum by using ammonium cerium sulfate. Background technique [0002] Fucoxanthin is a class of orange-yellow carotenoids mainly found in edible brown algae. Its chemical structure is a special polyene chain (conjugated double bond, epoxy group and conjugated carbonyl group with oxidation resistance). It is because of its structure that fucoxanthin is also considered as a class of lutein. Fucoxanthin plays an extremely important role in the treatment of human diseases. In the anti-obesity, anti-cancer and other diseases, the research on its mechanism of action also has a certain foundation. [0003] Phaeodactylum tricornutum has been paid attention and researched because of its ability to synthesize high content of oil. Studies have found that fucoxanthin exists in Phaeodactylum tricornut...
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