Special culture medium for liquefaction of Flammulina velutipes and corresponding culture method

Abstract

The invention discloses a special liquefied strain medium for flammulina velutipes. The medium consists of the following components in parts by weight: 50-60 parts of corn starch, 1-2 parts of glucose, 5-10 parts of wheat cellulose, 25-30 parts of fine bran, 1-1.5 parts of a yeast extract, 1-1.5 parts of beef peptone, 2-3 parts of monopotassium phosphate, 1-1.5 parts of magnesium sulfate and 100 parts of water. Furthermore, the invention also provides a flammulina velutipes liquefied strain culture method by virtue of the medium, and the method sequentially comprises the following steps: sterilizing the special liquefied strain medium for flammulina velutipes; inoculating flammulina velutipes mother strain to the sterilized medium under a sterile condition; carrying out dark culture at 17-19 DEG C for 19-21d so as to obtain a special liquefied solid strain; and liquefying the special liquefied solid strain so as to obtain the flammulina velutipes liquefied strain.

Description

technical field [0001] The invention relates to a special strain formula and cultivation method of Flammulina velutipes liquefaction. Background technique [0002] Edible fungus is one of the advantageous industries with the most modern agricultural development characteristics in China. This is not only because China is the world's largest producer and consumer of edible fungi, with a wide range of edible fungus production, but more importantly, the edible fungus industry is a good carrier and prominent position in the industrialization of biotechnology and the sustainable development of agriculture. At present, my country's edible fungus industry system has many factors that are not suitable for the global market economy, the most important of which are the intensification of industrial operations, low degree of specialization, backward production factors, imperfect production technology, and lack of key technology support. The most representative one is the intensive and e...

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Problems solved by technology

Remarks: The liquid fermentation strain process theoretically has a high purity of the strain, and the bacterial contamination rate of large-scale fermentation production is between 1% and 5% (in a 3-stage fermenter), and the bacterial contamination (mainly bacterial) is often In the later stage of fermentation, it is difficult to find out by conventional on-line inspection, which leads to production accidents caused by the inoculation of liquid fermentation strains and the contamination of large quantities of mushroom bags, which is a painful lesson.

In addition, because the cultivation of liquid strains requires nutrients such as high-concentration organic nitrogen and sugar sources, these ingredients remain in the bacterial liquid and are inserted into the fruiting bag. Since bacteria are inevitably brought in during operation, the residual nutrients It becomes a breeding ground for miscellaneous bacteria, which increases the risk of contamination after inoculation of the fruiting bag

This is the main problem that liquid fermentation strains cannot be applied to the production of edible fungi on a large scale

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