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A kind of method for measuring the concentration of sclareol in plasma

A technology for sclareol and plasma, which is applied in measuring devices, instruments, scientific instruments, etc., to achieve the effects of short time, strong specificity and small sample consumption

Inactive Publication Date: 2016-03-16
WUXI PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there is no literature report on the blood drug concentration detection method of sclareol. The present invention establishes the determination method of sclareol in rat plasma for the first time, and explores the drug metabolism of sclareol by this method Sexual research, laying a solid methodological foundation for its preclinical pharmacokinetic research

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  • A kind of method for measuring the concentration of sclareol in plasma
  • A kind of method for measuring the concentration of sclareol in plasma
  • A kind of method for measuring the concentration of sclareol in plasma

Examples

Experimental program
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Embodiment

[0026] Example: Determination of Sclareol Concentration in Rat Plasma

[0027] Experimental Materials and Instruments

[0028] Sclareol: purity>98%, batch number: MUST-14072810, purchased from Chengdu Mansite Biotechnology Co., Ltd.; internal standard: nitroazepam, batch number: 9201, purchased from the National Narcotics Laboratory; methanol (CNW company ), batch number: HX080973; ammonium acetate (Sinopharm Chemical Reagent Co., Ltd.), batch number: F20090520; water was made by ultrapure water (Milli-Q pure water machine).

[0029] Liquid chromatography system: ACCELAautosampler, ACCELA1250pump, American ThermoFisher Company; MS / MS system: TSQQuantumAccess triple quadrupole tandem mass spectrometer, equipped with electrospray ionization ESI ion source, American ThermoFisher Company; data acquisition: LCQUAN TM 2.6 software, American ThermoFisher company; XW-80A miniature vortex mixer, Shanghai Medical University Instrument Factory; Z233MK-2 high-speed centrifuge, German HER...

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Abstract

The invention relates to a method for determining concentration of sclareol in blood plasma and belongs to the technical field of biological detection. Nitrazepam is taken as an internal standard, a protein precipitation method is adopted, and the concentration of sclareol in the determined blood plasma is calculated by virtue of blood plasma sample pre-treatment, chromatograph, mass spectrometry and an internal standard method. The method for determining the concentration of sclareol in the blood plasma is rapid, accurate, high in sensitivity and easy to operate, and a methodological foundation is laid for drug metabolism dynamic research of sclareol in an animal body.

Description

technical field [0001] The invention relates to a method for measuring the concentration of sclareol in blood plasma, belonging to the technical field of biological detection. Background technique [0002] Sclareol (sclareol), also known as hard tail alcohol, is a ditertiary alcohol compound of the diterpenoid ditertiary alcohol isolated from Clary perilla, tobacco and other Salvia genus Labiatae plants. In vivo and in vitro studies have confirmed that it has anti-inflammatory, antibacterial, and bactericidal effects, and has varying degrees of killing effects on malignant tumor cells such as human leukemia cells, human colon cancer cells, and human breast cancer cells. It is a promising development prospect. New drug source compounds. Its structural formula is as follows: [0003] [0004] At present, there is no literature report on the blood drug concentration detection method of sclareol. The present invention establishes the determination method of sclareol in rat ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 黄凯张娜贺晴
Owner WUXI PEOPLES HOSPITAL
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