A Flexible Single Cell Separator and Separation and Culture Technology
A single-cell separation and separator technology, which is applied in specific-purpose bioreactors/fermenters, biochemical instruments, bioreactor/fermenter combinations, etc., to achieve low physical and chemical damage to cells, ensure cell growth, increase, and repeat Use high effect
Active Publication Date: 2017-01-04
HUNAN NENGRUN MEDICAL DIAGNOSIS TECH
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Problems solved by technology
[0005] In order to solve the problems still existing in the above-mentioned prior art, the present invention proposes a flexible single-cell separator and a method for separating and culturing
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specific Embodiment 1
[0026] A flexible single-cell separator, including but not limited to a micropipette, a culture bottle screw cap (3), a leather case (4), a conical suction tip (5), and the micropipette includes a micropipette fine end (1) And micropipette thick end (2), described micropipette thick end (2) connects the bottom of described tapered suction head (5), and described tapered suction pipe (5) tip top is elongated, and described holster (4) One end is fixed on the thick end of the micropipette (2), the other end of the leather sheath (4) is affixed to the screw cap (3) of the culture bottle, and the thick end of the micropipette (2) is embedded in the In the screw cap (3) of the culture bottle, the thick end of the micropipette (2) is connected to one end of the holster (4) to serve as a fixation and storage space, and the thin end (1) of the micropipette is used for cell Or for the absorption of cell clusters, the thin end (1) of the micropipette can be heated at a high temperature ...
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Abstract
The invention relates to a flexible single-cell separator. The flexible single-cell separator comprises, but not limited to, a micropipette, a culture flask screw cap, a leather sheath and a conical suction head, wherein the micropipette comprises a micropipette thin end and a micropipette thick end, the micropipette thick end is connected with the bottom of the conical suction head, the top of the tip of the conical suction head is long and thin, one end of the leather sheath is fixedly arranged at the micropipette thick end, the other end of the leather sheath is fixedly connected with the culture flask screw cap and the micropipette thick end is embedded in the culture flask screw cap. In addition, the invention also provides a single-cell separation culture method. According to the method, the separated single cell is cultured and amplified by virtue of a mixed solution of a used culture solution for culturing the cell and a fresh culture solution. The device has the beneficial effects that firstly, the device is simple in operation and low in cost; secondly, the operation can be performed under conventional visual conditions; thirdly, the separation of cells is purposeful; fourthly, a trace cell liquid can be transferred and the target cells are convenient to effectively separate after being diluted; and fifthly, low physical and chemical damages to cells are achieved.
Description
technical field [0001] The invention belongs to the field of biological cell engineering, and more specifically relates to a flexible single cell separator and separation and culture technology. Background technique [0002] In biological research, cells are the most important research carrier and object. Most cytological research requires functional modification and functional research on cells. In order to achieve research accuracy and functional consistency, monoclonal cells are considered The most effective cell form can ensure the consistency of the cell population and the stability and reliability of the results to the greatest possible extent. In the field of genetic research, monoclonal cells are the most commonly used research tools. [0003] Isolation of monoclonal cells is an essential link in the study of biological cells. In the study of gene function, the isolation of monoclonal cells is particularly important. [0004] The existing monoclonal cell separation ...
Claims
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IPC IPC(8): C12M1/24
Inventor 易银沙张万明戴燕山朱海强许澎刘彩云袁炳秋吴小宁
Owner HUNAN NENGRUN MEDICAL DIAGNOSIS TECH