High-oil-yield scenedesmus SDEC-13 and culture method and application thereof

A technology of SDEC-13 and Scenedesmus, which is applied in the field of high-yield Scenedesmus SDEC-13 and its cultivation, can solve the problems of high cultivation cost and insufficient high-quality algae strains, and achieve high-quality biodiesel, rich oil content, and reduce production cost effect

Active Publication Date: 2015-05-06
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the problem of insufficient fine algae strains and high culture cost for producing microalgae oil in the prior art, to provide a high-yield oil-yielding Scenedesmus and its cultivation method using industrial waste gas, that is, collecting water from natural water bodies In the same way, a kind of oleaginous microalgae with high oil content (27%) and easy open culture was obtained by separation and purification, and the culture method was established

Method used

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  • High-oil-yield scenedesmus SDEC-13 and culture method and application thereof
  • High-oil-yield scenedesmus SDEC-13 and culture method and application thereof
  • High-oil-yield scenedesmus SDEC-13 and culture method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0027] Acquisition and identification of algae species

[0028] 1. Sample collection

[0029] Microbe-rich water samples were collected from the artificial lake of Shandong Jianzhu University in Jinan City, Shandong Province in November 2012.

[0030] 2. Isolation, purification and screening of Scenedesmus SDEC-13

[0031] After the water samples were enriched and cultured, the algal cells were counted under a microscope. According to the counting results, the water sample was diluted to a concentration of algae cells of about 1 / 50 microliters. In a sterile environment, add 50 microliters of water sample to each well of the 96-well plate, and then add 200 microliters of BG 11 culture medium. The composition of BG 11 medium is: NaNO 3 1.5g / L, K 2 HPO 4 0.04g / L, MgSO 4 ·7H 2 O 0.075g / L, CaCl 2 2H 2 O 0.036g / L, Citric acid 0.006g / L, Ferric ammonium citrate 0.006g / L, EDTANa 0.001g / L, NaCO 30.02g / L, A5trace metal solution 1ml / L. The A5trace metal solution was composed of ...

Embodiment 2

[0044] In order to investigate the behavior of Scenedesmus SDEC-13 in high concentration CO 2 and harmful gas NO growth rate and the ability to tolerate harmful gases, use the above-mentioned SE medium to carry out aerated culture of Scenedesmus SDEC-13, and its composition and composition are as follows: NaNO 3 2.5gL -1 , K 2 HPO 4 0.075gL -1 ,MgSO 4 ·7H 2 O 0.075g L -1 ,CaCl 2 2H 2 O 0.025g L -1 ,KH 2 PO 4 0.175gL -1 ,NaCl 0.025g L -1 , Soil extract 40ml L -1 ,FeCl 3 ·6H 2 O 0.005g L -1 , Fe-EDTA 1ml L -1 ,H 3 BO 3 2.86mg·L -1 , MnCl 2 4H 2 O 1.86mg·L -1 , ZnSO 4 ·7H 2 O 0.22mg·L -1 , Na 2 MoO4·2H 2 O 0.39mg·L -1 , CuSO 4 ·5H 2 O 0.08mg·L -1 , Co(NO 3 ) 2 ·6H 2 O 0.05mg·L -1 , the medium is a carbon-free medium, so the growth of Scenedesmus can only use the CO in the aeration 2 . Inoculate fresh Scenedesmus SDEC-13 algae in the medium, and control the inoculation concentration to be about 0.5×10 6 per liter, the aeration components are ...

Embodiment 3

[0049] In order to investigate the behavior of Scenedesmus SDEC-13 in high concentration CO 2 and the ability to produce biodiesel during the cultivation of harmful gas NO, utilize the algae powder obtained in Example 2 to measure its total oil content and the composition of fatty acid methyl esters.

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Abstract

The invention discloses high-oil-yield scenedesmus SDEC-13 and a culture method and an application thereof. An algal strain disclosed by the invention is scenedesmus quadricauda SDEC-13, and can grow by virtue of high-concentration CO2; a gene sequence is submitted to a GenBank database; the number is KF999644; the algal specie is preserved at the China Center for Type Culture Collection in November 2014; and the preservation number is CCTCC M4014498. According to the algal specie disclosed by the invention, high-concentration CO2 in the waste gas can be utilized to reduce the culture cost. The scenedesmus disclosed by the invention is rich in excellent oil; the total oil content can reach 27.2% of dry weight; the saturated fatty acid content in fatty acid can be up to 82.87%; the content of the fatty acid of which the carbon chain length is 16-18 in fatty acid components can be up to over 98%; and the scenedesmus is suitable for being used as a raw material for producing biodiesel.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, in particular to a high-yielding Scenedesmus SDEC-13 and its cultivation method and application. Background technique [0002] Energy shortage and excessive emission of carbon dioxide are two prominent environmental problems in the world today. [0003] With the rapid development of society and economy, the consumption of primary energy continues to increase, and the energy gap is expanding year by year. At the same time, due to the burning of fossil fuels, excessive carbon dioxide is emitted into the atmosphere, leading to the global greenhouse effect. As a renewable energy source, biodiesel has the potential to solve both problems. Algae is a very efficient feedstock for biodiesel production. Its advantages are: 1. The growth rate is fast, and it can be harvested in more than ten days; 2. It can be cultivated in wastewater that cannot grow food, and does not occupy the area of ​​arable...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C02F3/32B01D53/84B01D53/62C12P7/64C12R1/89
CPCB01D53/62B01D53/84C02F3/322C12N1/12C12P7/6463C12P7/649C12N1/125C12R2001/89Y02E50/10Y02P20/59Y02A50/20
Inventor 裴海燕张硕韩琳祁峰
Owner SHANDONG UNIV
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