High-oil-yield scenedesmus SDEC-13 and culture method and application thereof
A technology of SDEC-13 and Scenedesmus, which is applied in the field of high-yield Scenedesmus SDEC-13 and its cultivation, can solve the problems of high cultivation cost and insufficient high-quality algae strains, and achieve high-quality biodiesel, rich oil content, and reduce production cost effect
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Embodiment 1
[0027] Acquisition and identification of algae species
[0028] 1. Sample collection
[0029] Microbe-rich water samples were collected from the artificial lake of Shandong Jianzhu University in Jinan City, Shandong Province in November 2012.
[0030] 2. Isolation, purification and screening of Scenedesmus SDEC-13
[0031] After the water samples were enriched and cultured, the algal cells were counted under a microscope. According to the counting results, the water sample was diluted to a concentration of algae cells of about 1 / 50 microliters. In a sterile environment, add 50 microliters of water sample to each well of the 96-well plate, and then add 200 microliters of BG 11 culture medium. The composition of BG 11 medium is: NaNO 3 1.5g / L, K 2 HPO 4 0.04g / L, MgSO 4 ·7H 2 O 0.075g / L, CaCl 2 2H 2 O 0.036g / L, Citric acid 0.006g / L, Ferric ammonium citrate 0.006g / L, EDTANa 0.001g / L, NaCO 30.02g / L, A5trace metal solution 1ml / L. The A5trace metal solution was composed of ...
Embodiment 2
[0044] In order to investigate the behavior of Scenedesmus SDEC-13 in high concentration CO 2 and harmful gas NO growth rate and the ability to tolerate harmful gases, use the above-mentioned SE medium to carry out aerated culture of Scenedesmus SDEC-13, and its composition and composition are as follows: NaNO 3 2.5gL -1 , K 2 HPO 4 0.075gL -1 ,MgSO 4 ·7H 2 O 0.075g L -1 ,CaCl 2 2H 2 O 0.025g L -1 ,KH 2 PO 4 0.175gL -1 ,NaCl 0.025g L -1 , Soil extract 40ml L -1 ,FeCl 3 ·6H 2 O 0.005g L -1 , Fe-EDTA 1ml L -1 ,H 3 BO 3 2.86mg·L -1 , MnCl 2 4H 2 O 1.86mg·L -1 , ZnSO 4 ·7H 2 O 0.22mg·L -1 , Na 2 MoO4·2H 2 O 0.39mg·L -1 , CuSO 4 ·5H 2 O 0.08mg·L -1 , Co(NO 3 ) 2 ·6H 2 O 0.05mg·L -1 , the medium is a carbon-free medium, so the growth of Scenedesmus can only use the CO in the aeration 2 . Inoculate fresh Scenedesmus SDEC-13 algae in the medium, and control the inoculation concentration to be about 0.5×10 6 per liter, the aeration components are ...
Embodiment 3
[0049] In order to investigate the behavior of Scenedesmus SDEC-13 in high concentration CO 2 and the ability to produce biodiesel during the cultivation of harmful gas NO, utilize the algae powder obtained in Example 2 to measure its total oil content and the composition of fatty acid methyl esters.
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