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A high-yielding Scenedesmus sdec-13 and its cultivation method and application

A SDEC-13, high-yield technology, applied in the direction of separation methods, chemical instruments and methods, and microbial-based methods, can solve the problems of insufficient fine algae strains and high cultivation costs, and achieve rich oil content, high biodiesel quality, and reduced The effect of training costs

Active Publication Date: 2019-04-30
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the problem of insufficient fine algae strains and high culture cost for producing microalgae oil in the prior art, to provide a high-yield oil-yielding Scenedesmus and its cultivation method using industrial waste gas, that is, collecting water from natural water bodies In the same way, a kind of oleaginous microalgae with high oil content (27%) and easy open culture was obtained by separation and purification, and the culture method was established

Method used

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  • A high-yielding Scenedesmus sdec-13 and its cultivation method and application
  • A high-yielding Scenedesmus sdec-13 and its cultivation method and application
  • A high-yielding Scenedesmus sdec-13 and its cultivation method and application

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Experimental program
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Effect test

Embodiment 1

[0027] Acquisition and identification of algae species

[0028] 1. Sample collection

[0029] Microbe-rich water samples were collected from the artificial lake of Shandong Jianzhu University in Jinan City, Shandong Province in November 2012.

[0030] 2. Isolation, purification and screening of Scenedesmus SDEC-13

[0031] After the water samples were enriched and cultured, the algal cells were counted under a microscope. According to the counting results, the water sample was diluted to a concentration of algae cells of about 1 / 50 microliters. In a sterile environment, add 50 microliters of water sample to each well of the 96-well plate, and then add 200 microliters of BG 11 culture medium. The composition of BG 11 medium is: NaNO 3 1.5g / L, K 2 HPO 4 0.04g / L, MgSO 4 ·7H 2 O 0.075g / L, CaCl 2 2H 2 O 0.036g / L, Citric acid 0.006g / L, Ferric ammonium citrate 0.006g / L, EDTANa 0.001g / L, NaCO 30.02g / L, A5trace metal solution 1ml / L. The A5trace metal solution was composed of ...

Embodiment 2

[0044] In order to investigate the behavior of Scenedesmus SDEC-13 in high concentration CO 2 and harmful gas NO growth rate and the ability to tolerate harmful gases, using the above SE medium for aerated culture of Scenedesmus SDEC-13, its composition and composition are as follows: NaNO 3 2.5gL -1 , K 2 HPO 4 0.075gL -1 ,MgSO 4 ·7H 2 O 0.075g L -1 ,CaCl 2 2H 2 O 0.025g L -1 ,KH 2 PO 4 0.175gL -1 ,NaCl 0.025g L -1 , Soil extract 40ml L -1 ,FeCl 3 ·6H 2 O 0.005g L -1 , Fe-EDTA 1ml L -1 ,H 3 BO 3 2.86mg·L -1 , MnCl 2 4H 2 O 1.86mg·L -1 , ZnSO 4 ·7H 2 O 0.22mg·L -1 , Na 2 MoO4·2H 2 O 0.39mg·L -1 , CuSO 4 ·5H 2 O 0.08mg·L -1 , Co(NO 3 ) 2 ·6H 2 O 0.05mg·L -1 , the medium is a carbon-free medium, so the growth of Scenedesmus can only use the CO in the aeration 2 . Inoculate fresh Scenedesmus SDEC-13 algae in the medium, and control the inoculation concentration to be about 0.5×10 6 per liter, the aeration components are air (CO 2 Concentrat...

Embodiment 3

[0049] In order to investigate the behavior of Scenedesmus SDEC-13 in high concentration CO 2 and the ability to produce biodiesel during the cultivation of harmful gas NO, utilize the algae powder obtained in Example 2 to measure its total oil content and the composition of fatty acid methyl esters.

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Abstract

The invention discloses a high-yield oil-yield Scenedesmus SDEC-13 and its cultivation method and application. The algal strain of the invention is Scenedesmus quadricauda SDEC-13, which can utilize high-concentration CO 2 grow. Its gene sequence has been submitted to the GenBank database, numbered KF999644. The algae species have been preserved in China Center for Type Culture Collection in November 2014, and its preservation number is CCTCC M 2014498. The algal species provided by the invention can utilize the high concentration of CO in the waste gas 2 Thereby reducing the cost of cultivation. Scenedesmus of the present invention is rich in high-quality oil, the total oil content can reach 27.2% of the dry weight, the saturated fatty acid content in the fatty acid can reach 82.87%, and among the fatty acid components, the fatty acid content with a carbon chain length of 16-18 can reach more than 98%. , suitable as a raw material for the production of biodiesel.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, in particular to a high-yielding Scenedesmus SDEC-13 and its cultivation method and application. Background technique [0002] Energy shortage and excessive emission of carbon dioxide are two prominent environmental problems in the world today. [0003] With the rapid development of society and economy, the consumption of primary energy continues to increase, and the energy gap is expanding year by year. At the same time, due to the burning of fossil fuels, excessive carbon dioxide is emitted into the atmosphere, leading to the global greenhouse effect. As a renewable energy source, biodiesel has the potential to solve both problems. Algae is a very efficient feedstock for biodiesel production. Its advantages are: 1. The growth rate is fast, and it can be harvested in more than ten days; 2. It can be cultivated in wastewater that cannot grow food, and does not occupy the area of ​​arable...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C02F3/32B01D53/84B01D53/62C12P7/64C12R1/89
CPCB01D53/62B01D53/84C02F3/322C12N1/12C12P7/6463C12P7/649C12N1/125C12R2001/89Y02E50/10Y02P20/59Y02A50/20
Inventor 裴海燕张硕韩琳祁峰
Owner SHANDONG UNIV
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