Applications of tumor-inhibiting factor Menin

A cell and liver cell technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc.

Active Publication Date: 2015-05-06
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, why Menin regulates hepatic lipogenesis remains largely unexplained
Therefore, the application of Menin in relation to liver cells is not known by researchers in this field.

Method used

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  • Applications of tumor-inhibiting factor Menin
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  • Applications of tumor-inhibiting factor Menin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] The specific embodiments of the present invention will be described below with reference to the drawings. It should be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention.

[0023] The experimental methods that do not indicate specific experimental conditions in the following examples usually follow conventional conditions such as those described in Sambrook et al., Molecular Cloning: Laboratory Manual (New York: Cold Spring Harbor Laboratory Press, 1989), or according to manufacturing The conditions suggested by the manufacturer.

[0024] Materials and Methods

[0025] Cell culture and reagents

[0026] HEK293T and HepG2 cells were purchased from the cell bank of the Type Culture Collection of the Chinese Academy of Sciences (CAS, Shanghai, China). The cells were cultured in Dulbecco's modified Eagle medium, supplemented with 10% fetal bovine serum (Gibco, Shanghai, China), 100 IU / ml penicill...

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PUM

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Abstract

The invention aims to provide an application of Menin in reduction of expression of an endogenous sterol regulatory element-binding protein 1c in HEK293T cells and liver cells, and is characterized by comprising a Menin over-expression step. Another objective of the invention is to provide an application of the Menin in inhibition of transcriptional activity of an HEK293T cell and liver X receptor LXR alpha, and is characterized by comprising a Menin over-expression step. Another objective of the invention is to provide an application of the Menin in inhibition of expression of a target gene of the HEK293T cell and liver X receptor LXR alpha, and is characterized by comprising steps of Menin over-expression and combination with the LXR alpha. According to the applications of the Menin, new research methods and reagents are provided for research and treatment of fatty liver, and a new way is provided for future treatment of fatty liver.

Description

Technical field [0001] The invention relates to the application of Menin protein and belongs to the field of biotechnology. Background technique [0002] Menin is encoded by the MEN1 gene and was originally identified as a suppressor of multiple endocrine tumors. Our previous report showed that Menin can enhance PPARα transcriptional activity and prevent liver triglyceride accumulation. [0003] Non-alcoholic fatty liver disease (NAFLD) or liver steatosis, characterized by the accumulation of triglycerides (TG) in liver cells, has become an important public health problem worldwide. In China, its prevalence reaches about 15% among adults. However, the underlying molecular mechanism of NAFLD process has not been clarified. [0004] In general, the TG homeostasis of the liver is strictly controlled by TG synthesis, β oxidation, and very low density lipoprotein (VLDL)-TG. The rate of hepatic fat production depends on a variety of lipogenic enzymes, which are caused by several nuclea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C12N15/861
Inventor 程鹏杨生生李刚金钢胡昊宋博
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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