Adenitis equorum disease inactivation vaccine and preparation method thereof

A technology of inactivated vaccine and Streptococcus equineides, applied in the direction of bacterial antigen components, antibacterial drugs, etc., can solve the problem of inability to prevent and achieve the effect of improving the protection rate

Inactive Publication Date: 2015-05-13
INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI +2
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since there is no domestically produced equine disease vaccine in our country so far, it...

Method used

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Embodiment 1

[0029] The present invention is achieved through the following technical solutions.

[0030] (1) Strain screening and standardization

[0031] The present invention selects Streptococcus equine epidemic Erdos epidemic strain (ES14), continuous culture detection, the culture is detected, and it is found that the 9th generation culture (ES14-09) meets the requirements of immune strains, and is used for vaccine preparation, and establishes a vaccine that meets the requirements of the vaccine. Prepared antigenic strains and test strains. The method of immune strain detection is:

[0032] A Morphology, biochemical characteristics and cultural characteristics Gram-positive cocci, the cells are round or oval, arranged in single, double or short chains. The bacteria cultured in the broth were in the form of long chains, and the isolated bacteria on the solid medium were in various forms, single or arranged in clusters.

[0033] Catalase reaction is negative, can ferment sucrose, gl...

experiment example 1

[0059] The inactivated vaccine of the equine gland blight prepared by the embodiment of the present invention 1 carries out the animal immunization experiment to a horse farm in the Ordos area, and the specific method is:

[0060] Foals within 6 months of age received 3ml intramuscular injection for the first time, 3ml intramuscular injection again 15 days later, and blood was collected to separate serum 15 days after the second injection.

[0061] The first intramuscular injection of 3ml vaccine is given to adult horses over one year old, followed by another intramuscular injection of 3ml 15 days later, and blood is collected to separate serum 15 days after the second injection.

[0062] Then its anti-virus protection rate is detected, and the method of anti-virus protection rate detection is:

[0063] (1) Take the test strain broth culture (bacteria content: 5 × 10 8 cfu / ml) 2ml; add an equal amount of horse serum neutralizing antibody to 1ml;

[0064] (2) Get 6 mice, of w...

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Abstract

The invention relates to the field of animal immune preparations, in particular to an adenitis equorum disease inactivation vaccine and a preparation method thereof. The adenitis equorum disease inactivation vaccine is prepared by taking a high-generation culture strain ES14-09 of an adenitis equorum streptococ cus Erdos epidemic strain ES14 as specific immunizing antigen through processes of seed batch preparation, culture, concentration, inactivation and adjuvant emulsification. The content of antigen bacteria of the adenitis equorum disease inactivation vaccine is 5*10<8>/ml. The adenitis equorum disease inactivation vaccine is high in protection rate and applicable to epidemic prevention of adenitis equorum diseases of equine animals.

Description

technical field [0001] The invention relates to the field of animal immune preparations, in particular to an inactivated equine glandular blight vaccine and a preparation method thereof. Background technique [0002] The Chinese nation has a long and deep relationship with horses, so Chinese people like horses and like to raise horses. The traditional horse breeding industry provides us with a variety of services, such as farming, national defense, transportation, riding, postal service, logging, etc. However, with the development of society and the popularization of mechanization, the use of horses as power has decreased, and horse breeding The industry has gradually transformed into a modern horse industry, that is, the non-service horse industry. It integrates culture, sports, competition, and leisure into one, and has become a new type of industry. It is rich in content and popular with the public, such as horse racing, equestrian sports, carriage Horse racing, performa...

Claims

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Application Information

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IPC IPC(8): A61K39/09A61P31/04
Inventor 郑润宽詹树柏郝文华赵世华耿万恒
Owner INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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