Synthetic method of 16-ene-20-hydroxy steroid compound
A technology of steroidal compounds and hydroxysteroids, applied in the direction of steroidal compounds, chemical instruments and methods, organic chemistry, etc., can solve the problems of no industrial production value, low selectivity, complicated reaction operation, etc., and reach the price of reagents Inexpensive, short reaction time, high chemoselectivity effect
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Embodiment 120
[0028] Example 120 Synthesis of α-hydroxy-pregna-4,16(17)-dien-3-one (II-1)
[0029]
[0030] In 3.12 grams (0.01 moles) of steroidal compound pregna-4,16(17)-diene-3,20-dione (English name 16-dehydro-Δ 4 -progestene-3,20-diones) (I-1), add 40 mL of glacial acetic acid, 20 mL of dichloromethane and 0.4 mL of water, stir at 0 ° C, then add 6.54 g (0.1 mole) of zinc powder, thin-layer chromatography The reaction was monitored by TLC until complete (about 3 hours). Post-treatment: filter out the zinc powder, spin off the dichloromethane, slowly pour the system into 400mL of water, stir for 30 minutes, filter, and dry in the air. Recrystallized with ethyl acetate to obtain 2.1 g of white solid, the filtrate was spin-dried and then column chromatographed to obtain 0.755 g of white solid, and finally 2.855 g of white solid 20α-hydroxy-pregna-4,16(17)-di En-3-one (English name is 16-dehydro-20α-hydroxy-Δ 4 -progesten-3-one) (II-1), the isolated yield is 90.8%.
[0031] Compoun...
Embodiment 220
[0032] Example 220 Synthesis of α-hydroxy-pregna-4,16(17)-dien-3-one (II-1)
[0033]
[0034] In 0.312 grams (0.001 moles) of steroidal compound pregnane-4,16(17)-diene-3,20-dione (English name is 16-dehydro-Δ 4 -progestene-3,20-diones) (I-1) was added 4 mL of acetic acid, stirred at 25°C, and then 0.654 g (0.01 mol) of zinc powder was added, and the reaction was monitored by thin layer chromatography (TLC) until it was complete. Post-treatment: filter out zinc powder, slowly add saturated sodium carbonate solution to the filtrate until no gas is produced. Separate the liquid, extract the aqueous phase twice with 15 mL ethyl acetate each time, combine the organic phases, then wash with water, wash with saturated brine, dry over anhydrous sodium sulfate, spin the filtrate and perform column chromatography to obtain 0.280 g of white solid 20α-hydroxyl -Pregnant-4,16(17)-diene-3-one (English name is 16-dehydro-20α-hydroxy-Δ 4 -progesten-3-one) (II-1), the isolated yield was ...
Embodiment 320
[0035] Example 320 Synthesis of α-hydroxy-pregna-4,16(17)-dien-3-one (II-1)
[0036]
[0037] In 0.312 grams (0.001 moles) of steroidal compound pregnane-4,16(17)-diene-3,20-dione (English name is 16-dehydro-Δ 4 -progestene-3,20-diones) (I-1) was added propionic acid 4mL, stirred at 25°C, then 0.654g (0.01mol) zinc powder was added, and the reaction was monitored by thin layer chromatography (TLC) until complete. Post-treatment: filter out zinc powder, slowly add saturated sodium carbonate solution to the filtrate until no gas is generated. Separate the liquid, extract the aqueous phase twice with 15 mL ethyl acetate each time, combine the organic phases, then wash with water, wash with saturated saline, dry over anhydrous sodium sulfate, spin the filtrate and perform column chromatography to obtain 0.265 g of white solid 20α-hydroxyl -Pregnant-4,16(17)-diene-3-one (English name is 16-dehydro-20α-hydroxy-Δ 4 -progesten-3-one) (II-I), the isolated yield was 84.3%.
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