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A kind of feeding method for improving the fermentation level of abamectin

A technology of abamectin and fermentation level, which is applied in the field of preparation of biological pesticides, and achieves the effects of obvious economic benefit and popularization value, small dosage, and simple and easy equipment.

Active Publication Date: 2018-10-09
QILU PHARMA INNER MONGOLIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems in feeding the fermentation process of the existing production of abamectin, the invention provides a method of feeding to improve the fermentation level of abamectin. The feeding method selects specific feeding and feeds at an appropriate time. Improve the fermentation titer, so that the production level of Abamectin has increased by 3-5%

Method used

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  • A kind of feeding method for improving the fermentation level of abamectin
  • A kind of feeding method for improving the fermentation level of abamectin
  • A kind of feeding method for improving the fermentation level of abamectin

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Embodiment 1

[0032] A kind of feeding method that improves the fermentation level of abamectin, the required abamectin fermentation feeding material is made up of starch, ammonium sulfate, yeast powder, amylase and water; Wherein, starch 1000kg, ammonium sulfate 2kg, brewer's yeast 20kg, α-amylase 1kg, water 8977kg, abamectin fermentation feeding total: 10 tons.

[0033] The feeding method is carried out as follows:

[0034] (1) Mix starch, ammonium sulfate, yeast powder, α-amylase and water and transport them to the feeding tank, raise the temperature to 75°C for liquefaction for 50 minutes, take a sample after sterilization and measure the reducing sugar 1.65g / 100ml; then raise the temperature Sterilize at 120°C for 30 minutes, and finally cool down to 75°C to obtain the liquefied feeding material after sterilization and set aside;

[0035] (2) The fermentation period of the fermenter reaches 168 hours, and the total sugar in the fermenter is measured to be less than 5.6g / 100ml, and the...

Embodiment 2

[0037]With the feeding method of the improvement of the Abamectin fermentation level of embodiment 1, the difference is:

[0038] Abamectin fermentation feed: starch 800kg, ammonium sulfate 2kg, brewer's yeast 20kg, α-amylase 0.8kg, water 9177.2kg

[0039] Step (1) Sampling after sterilization measures reducing sugar 1.58g / 100ml,

[0040] Step (2) The fermentation period of the fermenter is 216h, the total sugar in the fermenter is measured to be less than 5g / 100ml, and 2 tons of sterilized liquefaction supplement is added. After the supplement, the potency of the tank B1a is 6934μ / ml, compared with the control tank without feed and without any treatment, the titer was higher than that of the control tank by 264μ / ml when the tank was released.

Embodiment 3

[0042] With the feeding method of the improvement of the Abamectin fermentation level of embodiment 1, the difference is:

[0043] Abamectin fermentation feed: 600kg starch, 1kg ammonium sulfate, 10kg beer yeast, 0.5kg α-amylase, 9388.5kg water

[0044] Step (1) Sampling after sterilization measures reducing sugar 1.58g / 100ml,

[0045] Step (2) The fermentation cycle of the fermenter reaches 230 hours, and the total sugar in the fermenter is measured to be less than 4.5g / 100ml, and the liquefaction supplement after sterilization is added; After the liquefaction feeding, the titer of the tank B1a after the feeding was 7056 μ / ml, compared with the control tank without feeding and no treatment, the titer was higher than that of the control tank 218 μ / ml.

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Abstract

The invention relates to a feed feeding method for improving the fermentation level of abamectin. The starch concentration is 6-10%, the ammonium sulfate concentration is 0.01-0.05%, the yeast concentration is 0.1-0.5%, and the amylase concentration is 0.002-0.01. % of the mixture is feeding, and feeding is carried out in the middle and late stages of fermentation. This feeding method ensures the appropriate concentration of carbon source and nitrogen source in the material tank, and the appropriate timing of feeding, so that the production level of abamectin has been improved by 3 ‑5%, which effectively improves the level of fermentation production, and does not affect the internal quality of the product itself and downstream products, and has obvious economic benefits and promotional value.

Description

Technical field: [0001] The invention relates to a preparation method of biological pesticides, in particular to a feeding method for improving the fermentation level of abamectin. Background technique: [0002] Abamectin, the English name Avermectins, is a class of sixteen-membered macrolide compounds with insecticidal, acaricidal and nematicidal activities first developed by Omura Zhi of Kitasato University in Japan and Merck Company of the United States. Dual-purpose characteristics, produced by fermentation of Streptomyces avermitilis in Streptomyces griseus. Abamectin is a common pesticide formulation widely used in agriculture as an insecticide for the control of Brassicaceae diamondback moth, pear tree psyllid, cotton spider mite, citrus leaf miner rust tick, cotton boll Insects, apple tree peach borer and two-spotted spider mite, cabbage caterpillar, etc. The fermentation of abamectin is a heat-generating process. As the temperature rises, the water in the fermenta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/62C12R1/865
Inventor 王磊聂立强
Owner QILU PHARMA INNER MONGOLIA