Colloidal gold and light-emitting quantum dot dimer based selectable second-level sensitivity lateral chromatography rapid detection method

Abstract

The invention provides a colloidal gold and light-emitting quantum dot dimer based selectable second-level sensitivity lateral chromatography rapid detection method. when the requirement for the sensitivity is low, red reflection light of colloidal gold is utilized for detection under visible light; when the requirement for the sensitivity is high or colloidal gold cannot be distinguished, fluorescent light of the quantum dot is utilized for detection under an ultraviolet lamp; a nitrocellulose membrane is used as a carrier, liquid dropwise added at one end of a membrane strip is seeped and migrated to the other end of the membrane strip under the capillary action of a microporous membrane, antigen antibody combination, the color of the colloidal gold and the antigen fluorescence detection of the quantum dot are utilized; a test paper comprises a water absorption pad 1, a combination pad, a detection line, a quality control line, a water absorption pad, a bottom plate, and a nitrocellulose membrane, wherein the water absorption pad 1, the combination pad, the nitrocellulose membrane and a water absorption pad 2 are sequentially bonded on the bottom plate from one side of the bottom plate to the other side of the bottom plate, the detection line and the quality control line are arranged on the nitrocellulose membrane, the detection result is accurate and sensitive, and the method can realize selectable detection of macro molecular or micro molecular substances.

Description

Technical field: [0001] The invention relates to the detection fields of medical detection, food quality detection, environmental monitoring, agriculture and animal husbandry detection, entry-exit inspection and quarantine, etc., taking melamine as an example to describe the preparation method, detection method and application of optional secondary sensitivity detection test paper. Background technique: [0002] Optional secondary sensitivity detection test paper is developed based on colloidal gold immunochromatography test paper. Colloidal gold immunochromatography test paper is a rapid diagnostic technology emerging abroad in recent years. Its principle is to apply colloidal gold rapid detection technology to antigen Antibody reaction, the specific antibody is first fixed on a certain zone of the nitrocellulose membrane, when one end of the dry nitrocellulose membrane is immersed in the sample, due to the capillary action of the microporous membrane, the sample will move f...

AI Technical Summary

Problems solved by technology

[0003] At present, the detection method of colloidal gold immunochromatography test paper mainly relies on the observation and judgment of human eyes, and the detection sensitivity can only be at the nanogram level (ng); The color contrast of the test zone is not obvious, it is difficult to judge visually, and there are problems of false positives and false negatives; therefore, it is urgent to develop a detection test paper that can achieve high and low sensitivity on the same test paper

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