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Low field NMR (nuclear magnetic resonance)-based magnetic bead concentration detection method and application thereof

A low-field nuclear magnetic resonance and magnetic bead technology, applied in the field of molecular biology, can solve the problems of inability to adjust, consumers cannot know the accuracy of the manufacturer, and achieve the effect of saving costs, preventing failures and losses, and optimizing experimental results.

Inactive Publication Date: 2014-10-29
张祥林
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

That is to say, consumers have no way of knowing the accuracy of the information provided by the manufacturer, nor can they make post-adjustments according to work needs.

Method used

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  • Low field NMR (nuclear magnetic resonance)-based magnetic bead concentration detection method and application thereof
  • Low field NMR (nuclear magnetic resonance)-based magnetic bead concentration detection method and application thereof

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Embodiment 1

[0038] 1. Sample Preparation

[0039] 1.1 Sensitive area detection of magnetic bead concentration change:

[0040] Wash the 10mg / ml magnetic beads with a volume of 1ml twice with buffer, and the magnetic beads washing method is as follows:

[0041] 1.1.11ml of the magnetic bead suspension (including the buffer that comes with the magnetic beads) is briefly centrifuged and carefully transferred to a clean 1.5ml EP tube. After another brief centrifugation, place it on a magnetic stand to absorb the magnetic beads.

[0042] 1.1.2 Carefully suck out the supernatant and store it in another clean EP tube, being careful not to get the precipitate.

[0043] 1.1.3 Add 500 μl sterilized deionized water, vortex and mix well, then put the centrifuge tube on the magnetic stand to absorb the magnetic beads. After 5 minutes, the supernatant was aspirated and discarded, being careful not to get the magnetic beads.

[0044] 1.1.4 Repeat step 1.3

[0045] 1.1.5 Add 1ml of buffer solution, v...

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Abstract

The invention discloses a low field NMR (nuclear magnetic resonance)-based magnetic bead concentration detection method and application thereof, the method comprises the following steps: (1) preparing a series of concentrations of magnetic bead suspensions, using measured NMR relaxation time T as y-coordinates, using the magnetic bead suspension concentrations as x-coordinates to draw a magnetic bead concentration-relaxation time curve to obtain a magnetic bead concentration change sensitive area; (2) preparing a series of magnetic bead suspension standard samples with the concentrations in the sensitive area, using measured relaxation time Tsample as y-coordinates, using the magnetic bead suspension standard sample concentrations as x-coordinates to draw a sensitive area magnetic bead concentration-relaxation time standard curve; (3) preparing a magnetic bead suspension sample to be tested, diluting to the concentration change sensitive area, detecting the relaxation time Ttest, and obtaining the concentration of the magnetic bead suspension sample to be tested from the sensitive area magnetic bead concentration-relaxation time standard curve. The method can rapidly detect the magnetic bead concentration, provides effective and accurate quality control information to experimenters, and has important significance for the effective use and sorting and efficiency evaluation of magnetic beads.

Description

technical field [0001] The invention relates to molecular biology technology, in particular to a method for detecting the concentration of magnetic beads by using low-field nuclear magnetic resonance and its application. Background technique [0002] High-throughput and automated extraction of nucleic acids can be achieved by coating magnetic beads (such as silicon, amino, carboxyl, etc.). , prenatal diagnosis, etc., has formed a mature industry. Compared with the traditional DNA extraction method, which is time-consuming and labor-intensive and the purity is not high, the advantages of magnetic bead method DNA extraction are becoming more and more obvious. In addition, immunomagnetic beads (ImpetiCbead, IMB referred to as magnetic beads) combine the unique advantages of solidified reagents with the high specificity of immunological reactions. Based on immunology, they penetrate into pathological, physiological, pharmacological, microbial, biochemical and molecular genetics...

Claims

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Application Information

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IPC IPC(8): G01N24/08
Inventor 张祥林
Owner 张祥林
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